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验证数据展示

  • IF Staining of HepG2 using 83955-4-RR

    IF Staining of HepG2 using 83955-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TLE3 antibody (83955-4-RR, Clone: 240680G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83955-4-RR

    Affinity and Kinetic Characterization of 83955-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83955-4-RR against Human TLE3 were performed. The affinity constant is 0.134 nM.

TLE3 Recombinant monoclonal antibody

TLE3 Uni-rAb® Recombinant Antibody for IF/ICC, ELISA
Cat No. 83955-4-RR

产品说明书

CloneNo. 240680G3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, ELISA

别名

GRG3, GRG 3, ESG3, ESG, Enhancer of split groucho-like protein 3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy TLE3 Ready-To-Use IHC Kit. TLE3 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83955-4-RR targets TLE3 in IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag1930

Product name: Recombinant human TLE3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC015729

Sequence: MYPQGRHPAPHQPGQPGFKFTVAESCDRIKDEFQFLQAQYHSLKVEYDKLANEKTEMQRHYVMYYEMSYGLNIEMHKQTEIAKRLNTILAQIMPFLSQEHQQQVAQAVERAKQVTMTELNAIIGQQQLQAQHLSHATHGPPVQLPPHPSGLQPPGIPPVTGSSSGLLALGALGSQAHLTVKDEKNHHELDHRERESSANNSVSPSESLRASEKHRGSADYSMEAKKRKAEEKDSLSRYDSDGDKSDDLVVDVSNEDPATPRVSPAHSPPENGLDKARSLKKDAPTSPASVASSSSTPSSKTKDLGHNDKSSTPGLKSNTPTPRNDAPTPGTSTTPGLRSMPGKPPGMDPI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila)
别名 GRG3, GRG 3, ESG3, ESG, Enhancer of split groucho-like protein 3
计算分子量 772 aa, 83 kDa
GenBank蛋白编号BC015729
基因名称 TLE3
Gene ID (NCBI) 7090
RRIDAB_3671534
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ04726
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TLE3, also named as KIAA1547 and ESG3, belongs to the WD repeat Groucho/TLE family. It is a transcriptional corepressor that binds to a number of transcription factors. It inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. TLE3 is a member of the transducin-like enhancer of split (TLE) family of proteins that have been implicated in the tumorgenesis and classification of sarcomas. It is a candidate biomarker of response to taxane

实验方案

Product Specific Protocols
IF protocol for TLE3 antibody 83955-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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