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验证数据展示

  • WB analysis using 83846-1-RR (same clone as 83846-1-PBS)

    WB analysis using 83846-1-RR (same clone as 83846-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83846-1-RR (USP53 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83846-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 83846-1-RR (same clone as 83846-1-PBS)

    IHC staining of mouse testis using 83846-1-RR (same clone as 83846-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83846-1-RR (USP53 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83846-1-PBS in a different storage buffer formulation.

  • IHC staining of rat liver using 83846-1-RR (same clone as 83846-1-PBS)

    IHC staining of rat liver using 83846-1-RR (same clone as 83846-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 83846-1-RR (USP53 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83846-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00821-1

    Cytometric bead array standard curve of MP00821-1

    Cytometric bead array standard curve of MP00821-1, USP53 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83846-1-PBS. Detection antibody: 83846-2-PBS. Standard: Ag28923. Range: 0.78-100 ng/mL.

  • Cytometric bead array standard curve of MP00821-2

    Cytometric bead array standard curve of MP00821-2

    Cytometric bead array standard curve of MP00821-2, USP53 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83846-1-PBS. Detection antibody: 83846-3-PBS. Standard: Ag28923. Range: 1.56-100 ng/mL.

  • Affinity and Kinetic Characterization of 83846-1-RR

    Affinity and Kinetic Characterization of 83846-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83846-1-RR against Human USP53 were performed. The affinity constant is 0.878 nM.

USP53 Recombinant antibody, PBS Only (Capture)

USP53 Uni-rAbTM Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 83846-1-PBS

产品说明书

CloneNo. 240971A6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Cytometric bead array, Indirect ELISA

别名

KIAA1350, Inactive ubiquitin-specific peptidase 53, Inactive ubiquitin carboxyl-terminal hydrolase 53, 240971A6

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83846-1-PBS targets USP53 as part of a matched antibody pair:

MP00821-1: 83846-1-PBS capture and 83846-2-PBS detection (validated in Cytometric bead array)

MP00821-2: 83846-1-PBS capture and 83846-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 USP53 fusion protein Ag28923 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ubiquitin specific peptidase 53
别名 KIAA1350, Inactive ubiquitin-specific peptidase 53, Inactive ubiquitin carboxyl-terminal hydrolase 53, 240971A6
计算分子量 121 kDa
观测分子量120-130 kDa
GenBank蛋白编号NM_019050
基因名称 USP53
Gene ID (NCBI) 54532
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ70EK8
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ubiquitin specific peptidase 53 (USP53) was originally discovered in cholestasis, and its tumor suppressive effects have recently been reported in various cancers. Zhao et al. showed that USP53 interacted with FKBP51 in lung adenocarcinoma cells, and induced apoptosis via the AKT pathway. Furthermore, USP53 inhibited the progression of clear cell renal cell carcinoma by suppressing the nuclear factor κB (NF‐κB) pathway. (PMID: 35654790)