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验证数据展示

  • WB analysis using 83846-1-RR

    WB analysis using 83846-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83846-1-RR (USP53 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 83846-1-RR

    IHC staining of mouse testis using 83846-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83846-1-RR (USP53 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat liver using 83846-1-RR

    IHC staining of rat liver using 83846-1-RR

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 83846-1-RR (USP53 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83846-1-RR

    Affinity and Kinetic Characterization of 83846-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83846-1-RR against Human USP53 were performed. The affinity constant is 0.878 nM.

USP53 Recombinant antibody

USP53 Recombinant Antibody for WB, IHC, ELISA
Cat No. 83846-1-RR

产品说明书

CloneNo. 240971A6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

KIAA1350, Inactive ubiquitin-specific peptidase 53, Inactive ubiquitin carboxyl-terminal hydrolase 53, 240971A6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, A431 cells, HeLa cells, HepG2 cells
Positive IHC detected inrat liver tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83846-1-RR targets USP53 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 USP53 fusion protein Ag28923 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ubiquitin specific peptidase 53
别名 KIAA1350, Inactive ubiquitin-specific peptidase 53, Inactive ubiquitin carboxyl-terminal hydrolase 53, 240971A6
计算分子量 121 kDa
观测分子量120-130 kDa
GenBank蛋白编号NM_019050
基因名称 USP53
Gene ID (NCBI) 54532
RRIDAB_3671432
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ70EK8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ubiquitin specific peptidase 53 (USP53) was originally discovered in cholestasis, and its tumor suppressive effects have recently been reported in various cancers. Zhao et al. showed that USP53 interacted with FKBP51 in lung adenocarcinoma cells, and induced apoptosis via the AKT pathway. Furthermore, USP53 inhibited the progression of clear cell renal cell carcinoma by suppressing the nuclear factor κB (NF‐κB) pathway. (PMID: 35654790)

实验方案

Product Specific Protocols
WB protocol for USP53 antibody 83846-1-RRDownload protocol
IHC protocol for USP53 antibody 83846-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols