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验证数据展示

  • WB analysis of U-87 MG using 83767-1-RR

    WB analysis of U-87 MG using 83767-1-RR

    U-87 MG cells were subjected to SDS PAGE followed by western blot with 83767-1-RR (TNC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 83767-1-RR

    IHC staining of human stomach cancer using 83767-1-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83767-1-RR (TNC/Tenascin-C antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TNC/Tenascin-C antibody included
  • IHC staining of human stomach cancer using 83767-1-RR

    IHC staining of human stomach cancer using 83767-1-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83767-1-RR (TNC/Tenascin-C antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TNC/Tenascin-C antibody included
  • IF Staining of HCT 116 using 83767-1-RR

    IF Staining of HCT 116 using 83767-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HCT 116 cells using TNC/Tenascin-C antibody (83767-1-RR, Clone: 240844A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83767-1-RR

    Affinity and Kinetic Characterization of 83767-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83767-1-RR against Human TNC/Tenascin-C were performed. The affinity constant is below 1 pM.

TNC/Tenascin-C Recombinant monoclonal antibody

TNC/Tenascin-C Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83767-1-RR

产品说明书

CloneNo. 240844A2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

Tenascin, TNC, 240844A2, Glioma-associated-extracellular matrix antigen, GMEM

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inU-87 MG cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHCT 116 cells
Planning an IHC experiment? We recommend our IHCeasy TNC/Tenascin-C Ready-To-Use IHC Kit. TNC/Tenascin-C primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83767-1-RR targets TNC/Tenascin-C in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag27122

Product name: Recombinant human TNC protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 23-200 aa of BC151843

Sequence: GVLKKVIRHKRQSGVNATLPEENQPVVFNHVYNIKLPVGSQCSVDLESASGEKDLAPPSEPSESFQEHTVDGENQIVFTHRINIPRRACGCAAAPDVKELLSRLEELENLVSSLREQCTAGAGCCLQPATGRLDTRPFCSGRGNFSTEGCGCVCEPGWKGPNCSEPECPGNCHLRGRC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tenascin C
别名 Tenascin, TNC, 240844A2, Glioma-associated-extracellular matrix antigen, GMEM
计算分子量 2201 aa, 241 kDa
观测分子量220-350 kDa, 190-240 kDa
GenBank蛋白编号BC151843
基因名称 Tenascin C
Gene ID (NCBI) 3371
RRIDAB_3671361
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP24821
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tenascin-C (TNC) is a large hexameric extracellular matrix glycoprotein of the tenascin family (PMID: 21818551). It is a multimodular protein containing multiple epidermal growth factor (EGF)-like repeats and fibronectin type III (FN III) domains (PMID: 1719530). Tenascin-C is highly expressed during embryonic development, particularly in the developing central nervous system, around motile cells and at epithelial-mesenchymal interaction sites (PMID: 25738825). In adult tissues, the expression and the distribution of TNC are typically limited under normal physiological conditions. It is upregulated during injury, inflammation, regeneration, and cancer (PMID: 7694605; 25120494). Tenascin-C is a diverse protein that can produce different functions including regulating cell adhesion, migration and proliferation.

实验方案

Product Specific Protocols
IF protocol for TNC/Tenascin-C antibody 83767-1-RRDownload protocol
IHC protocol for TNC/Tenascin-C antibody 83767-1-RRDownload protocol
WB protocol for TNC/Tenascin-C antibody 83767-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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