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验证数据展示

  • WB analysis using 83628-1-RR

    WB analysis using 83628-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83628-1-RR (UBC12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • FC experiment of HEK-293 using 83628-1-RR

    FC experiment of HEK-293 using 83628-1-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug UBC12 Recombinant antibody (83628-1-RR, Clone:240477H5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83628-1-RR

    Affinity and Kinetic Characterization of 83628-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83628-1-RR against Human UBC12 were performed. The affinity constant is 2.45 nM.

UBC12 Recombinant monoclonal antibody

UBC12 Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83628-1-RR

产品说明书

CloneNo. 240477H5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

UBE2M, NEDD8 protein ligase, NEDD8 conjugating enzyme Ubc12, NEDD8 carrier protein, hUbc12

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, L02 cells, SMMC-7721 cells, A549 cells, HT-1080 cells
Positive FC (Intra) detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83628-1-RR targets UBC12 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag5984

Product name: Recombinant human UBC12 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-183 aa of BC058924

Sequence: MIKLFSLKQQKKEEESAGGTKGSSKKASAAQLRIQKDINELNLPKTCDISFSDPDDLLNFKLVICPDEGFYKSGKFVFSFKVGQGYPHDPPKVKCETMVYHPNIDLEGNVCLNILREDWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQRSMRGGYIGSTYFERCLK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)
别名 UBE2M, NEDD8 protein ligase, NEDD8 conjugating enzyme Ubc12, NEDD8 carrier protein, hUbc12
计算分子量 21 kDa
观测分子量 17-20 kDa
GenBank蛋白编号BC058924
基因名称 UBC12
Gene ID (NCBI) 9040
RRIDAB_3671241
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP61081
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UBE2M (Ubiquitin-conjugating enzyme E2 M), also called UBC12, accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction between UBE2M and the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation (PMID: 10207026; 15361859).

实验方案

Product Specific Protocols
FC protocol for UBC12 antibody 83628-1-RRDownload protocol
WB protocol for UBC12 antibody 83628-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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