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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 83624-1-RR (same clone as 83624-1-PBS)

    WB analysis using 83624-1-RR (same clone as 83624-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83624-1-RR (HDAC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83624-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 83624-1-RR (same clone as 83624-1-PBS)

    IHC staining of mouse colon using 83624-1-RR (same clone as 83624-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83624-1-RR (HDAC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83624-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HDAC1 antibody included
  • IF Staining of HeLa using 83624-1-RR (same clone as 83624-1-PBS)

    IF Staining of HeLa using 83624-1-RR (same clone as 83624-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HDAC1 antibody (83624-1-RR, Clone: 240614A3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83624-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83624-1-RR (same clone as 83624-1-PBS)

    IF Staining of HeLa using 83624-1-RR (same clone as 83624-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HDAC1 antibody (83624-1-RR, Clone: 240614A3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83624-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83624-1-RR (same clone as 83624-1-PBS)

    FC experiment of HeLa using 83624-1-RR (same clone as 83624-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug HDAC1 Recombinant antibody (83624-1-RR, Clone:240614A3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83624-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83624-1-PBS

    Affinity and Kinetic Characterization of 83624-1-PBS

    Biolayer interferometry (BLl) kinetic assay of 83624-1-PBS against Human HDAC1 was performed. The affinity constant is below 1 pM.

  • Affinity and Kinetic Characterization of 83624-1-RR

    Affinity and Kinetic Characterization of 83624-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83624-1-RR against Human HDAC1 were performed. The affinity constant is below 1 pM.

HDAC1 Recombinant antibody, PBS Only

HDAC1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83624-1-PBS

产品说明书

CloneNo. 240614A3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

240614A3, EC:3.5.1.-, EC:3.5.1.98, GON 10, HD1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy HDAC1 Ready-To-Use IHC Kit. HDAC1 primary antibody included.

产品信息

83624-1-PBS targets HDAC1 in WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 HDAC1 fusion protein Ag0256 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 histone deacetylase 1
别名 240614A3, EC:3.5.1.-, EC:3.5.1.98, GON 10, HD1
计算分子量 55 kDa
观测分子量 58-66 kDa
GenBank蛋白编号BC000301
基因名称 HDAC1
Gene ID (NCBI) 3065
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13547
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex, which is responsible for gene expression silencing. It also plays an important role in the control of cell proliferation and differentiation by interacting with RB, p53 and other transcription factors. At least 4 classes of HDAC were identified. As a class I HDAC, HDAC 1 was primarily found in the nucleus.