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验证数据展示

  • WB analysis using 83569-1-RR

    WB analysis using 83569-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83569-1-RR (HIBCH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 83569-1-RR

    WB analysis of HepG2 using 83569-1-RR

    WB result of HIBCH antibody (83569-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HIBCH transfected HepG2 cells.

  • IF Staining of HepG2 using 83569-1-RR

    IF Staining of HepG2 using 83569-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HIBCH antibody (83569-1-RR, Clone: 240590A3 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • FC experiment of HepG2 using 83569-1-RR

    FC experiment of HepG2 using 83569-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug HIBCH Recombinant antibody (83569-1-RR, Clone:240590A3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using 83569-1-RR

    IF Staining of HepG2 using 83569-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HIBCH antibody (83569-1-RR, Clone: 240590A3 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • Affinity and Kinetic Characterization of 83569-1-RR

    Affinity and Kinetic Characterization of 83569-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83569-1-RR against Human HIBCH were performed. The affinity constant is 1.37 nM.

  • Featured Product
  • KD/KO验证

HIBCH Recombinant antibody

HIBCH Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83569-1-RR

产品说明书

CloneNo. 240590A3

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IF/ICC, FC (Intra), ELISA

别名

HIB-CoA hydrolase, HIB CoA hydrolase, EC:3.1.2.4, 3-hydroxyisobutyryl-coenzyme A hydrolase, 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, HeLa cells, rat liver tissue
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HIBCH Ready-To-Use IHC Kit. HIBCH primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83569-1-RR targets HIBCH in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, rat
免疫原 HIBCH fusion protein Ag6149 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 3-hydroxyisobutyryl-Coenzyme A hydrolase
别名 HIB-CoA hydrolase, HIB CoA hydrolase, EC:3.1.2.4, 3-hydroxyisobutyryl-coenzyme A hydrolase, 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial
计算分子量 43 kDa
观测分子量 38 kDa
GenBank蛋白编号BC067822
基因名称 HIBCH
Gene ID (NCBI) 26275
RRIDAB_3671182
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ6NVY1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HIBCH belongs to the enoyl-CoA hydratase/isomerase family. HIBCH has two isoforms with MW 43 kDa and 38 kDa. It is highly expressed in the liver and kidney, also detected in the heart, muscle and brain (at protein level), but not detected in the lung. Hydrolyzes 3-hydroxyisobutyryl-CoA (HIBYL-CoA), a saline catabolite. Has high activity toward isobutyryl-CoA. Could be an isobutyryl-CoA dehydrogenase that functions in valine catabolism. Also hydrolyzes 3-hydroxypropanoyl-CoA (PMID: 8824301).

实验方案

Product Specific Protocols
WB protocol for HIBCH antibody 83569-1-RRDownload protocol
IF protocol for HIBCH antibody 83569-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols