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验证数据展示

  • WB analysis using 83569-1-RR (same clone as 83569-1-PBS)

    WB analysis using 83569-1-RR (same clone as 83569-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83569-1-RR (HIBCH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83569-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 83569-1-RR (same clone as 83569-1-PBS)

    WB analysis of HepG2 using 83569-1-RR (same clone as 83569-1-PBS)

    WB result of HIBCH antibody (83569-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HIBCH transfected HepG2 cells. This data was developed using the same antibody clone with 83569-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83569-1-RR (same clone as 83569-1-PBS)

    IF Staining of HepG2 using 83569-1-RR (same clone as 83569-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HIBCH antibody (83569-1-RR, Clone: 240590A3 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83569-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 83569-1-RR (same clone as 83569-1-PBS)

    FC experiment of HepG2 using 83569-1-RR (same clone as 83569-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug HIBCH Recombinant antibody (83569-1-RR, Clone:240590A3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83569-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83569-1-RR (same clone as 83569-1-PBS)

    IF Staining of HepG2 using 83569-1-RR (same clone as 83569-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HIBCH antibody (83569-1-RR, Clone: 240590A3 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 83569-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00560-3

    Cytometric bead array standard curve of MP00560-3

    Cytometric bead array standard curve of MP00560-3, HIBCH Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83569-3-PBS. Detection antibody: 83569-1-PBS. Standard: Ag6149. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 83569-1-RR

    Affinity and Kinetic Characterization of 83569-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83569-1-RR against Human HIBCH were performed. The affinity constant is 1.37 nM.

  • Featured Product
  • KD/KO验证

HIBCH Recombinant antibody, PBS Only (Detector)

HIBCH Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83569-1-PBS

产品说明书

CloneNo. 240590A3

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

HIB-CoA hydrolase, HIB CoA hydrolase, EC:3.1.2.4, 3-hydroxyisobutyryl-coenzyme A hydrolase, 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy HIBCH Ready-To-Use IHC Kit. HIBCH primary antibody included.

产品信息

83569-1-PBS targets HIBCH as part of a matched antibody pair:

MP00560-3: 83569-3-PBS capture and 83569-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原 HIBCH fusion protein Ag6149 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 3-hydroxyisobutyryl-Coenzyme A hydrolase
别名 HIB-CoA hydrolase, HIB CoA hydrolase, EC:3.1.2.4, 3-hydroxyisobutyryl-coenzyme A hydrolase, 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial
计算分子量 43 kDa
观测分子量 38 kDa
GenBank蛋白编号BC067822
基因名称 HIBCH
Gene ID (NCBI) 26275
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ6NVY1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HIBCH belongs to the enoyl-CoA hydratase/isomerase family. HIBCH has two isoforms with MW 43 kDa and 38 kDa. It is highly expressed in the liver and kidney, also detected in the heart, muscle and brain (at protein level), but not detected in the lung. Hydrolyzes 3-hydroxyisobutyryl-CoA (HIBYL-CoA), a saline catabolite. Has high activity toward isobutyryl-CoA. Could be an isobutyryl-CoA dehydrogenase that functions in valine catabolism. Also hydrolyzes 3-hydroxypropanoyl-CoA (PMID: 8824301).