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验证数据展示

  • WB analysis using 83548-3-RR

    WB analysis using 83548-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83548-3-RR (GHITM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 83548-3-RR

    FC experiment of HeLa using 83548-3-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug GHITM Recombinant antibody (83548-3-RR, Clone:240541D3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83548-3-RR

    Affinity and Kinetic Characterization of 83548-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83548-3-RR against Human GHITM were performed. The affinity constant is 2.41 nM.

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GHITM Recombinant monoclonal antibody

GHITM Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83548-3-RR

产品说明书

CloneNo. 240541D3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), ELISA

别名

My021, MICS1, Growth hormone-inducible transmembrane protein, DERP2, Dermal papilla-derived protein 2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293T cells, SH-SY5Y cells, mouse liver tissue
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:600-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83548-3-RR targets GHITM in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag35170

Product name: Recombinant human GHITM protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-72 aa of BC010354

Sequence: MLAARLVCLRTLPSRVFHPAFTKASPVVKNSITKNQWLLTPSREYATKTRIGIRRGRTGQELKEAALEPSME

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 growth hormone inducible transmembrane protein
别名 My021, MICS1, Growth hormone-inducible transmembrane protein, DERP2, Dermal papilla-derived protein 2
计算分子量 345 aa, 37 kDa
观测分子量37 kDa
GenBank蛋白编号BC010354
基因名称 GHITM
Gene ID (NCBI) 27069
RRIDAB_3671167
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9H3K2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GHITM, also known as MICS1, TMBIM5 or DERP2, is a mitochondrial protein that localizes in the inner membrane. GHITM is involved in mitochondrial morphology in specific cristae structures and the apoptotic release of cytochrome c from the mitochondria (PMID: 18417609). The gene of GHITM maps to chromosome 10q23.1, and encodes a 345-amino-acid protein with a calculated molecular mass of 37 kDa.

实验方案

Product Specific Protocols
WB protocol for GHITM antibody 83548-3-RRDownload protocol
FC protocol for GHITM antibody 83548-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols