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验证数据展示

  • WB analysis of mouse placenta using 83367-2-RR (same clone as 83367-2-PBS)

    WB analysis of mouse placenta using 83367-2-RR (same clone as 83367-2-PBS)

    mouse placenta tissue were subjected to SDS PAGE followed by western blot with 83367-2-RR (Afp antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83367-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00395-4

    Sandwich ELISA standard curve of MP00395-4

    Sandwich ELISA standard curve of MP00395-4, Mouse AFP Monoclonal Matched Antibody Pair - PBS only. 83367-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0670. 83367-2-PBS was HRP conjugated as the detection antibody. Range: 78.125-5000 pg/mL.

  • Sample test of MP00395-4

    Sample test of MP00395-4

    Serum of sixteen individual healthy mice were measured. The mouse AFP concentration of detected samples was determined to be113.4 ng/mL with a range of 66.5-245.7 ng/mL.

  • Cytometric bead array standard curve of MP00395-2

    Cytometric bead array standard curve of MP00395-2

    Cytometric bead array standard curve of MP00395-2, MOUSE AFP Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83367-4-PBS. Detection antibody: 83367-2-PBS. Standard: Eg0670. Range: 0.625-40 ng/mL.

  • Affinity and Kinetic Characterization of 83367-2-RR

    Affinity and Kinetic Characterization of 83367-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83367-2-RR against Mouse Afp were performed. The affinity constant is 1.69 nM.

Mouse Afp Recombinant antibody, PBS Only (Detector)

Afp Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83367-2-PBS

产品说明书

CloneNo. 240232C7

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

Alpha-fetoprotein, Alpha-fetoglobulin, Alpha-1-fetoprotein, alpha fetoprotein, 240232C7

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83367-2-PBS targets Afp as part of a matched antibody pair:

MP00395-2: 83367-4-PBS capture and 83367-2-PBS detection (validated in Cytometric bead array)

MP00395-4: 83367-5-PBS capture and 83367-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 alpha fetoprotein
别名 Alpha-fetoprotein, Alpha-fetoglobulin, Alpha-1-fetoprotein, alpha fetoprotein, 240232C7
计算分子量67KD
观测分子量67-72 kDa
GenBank蛋白编号NM_007423.4
基因名称 Afp
Gene ID (NCBI) 11576
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP02772
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Alpha-fetoprotein (AFP) is one of the earliest proteins to be synthesised by the embryonic liver. The synthesis of AFP decreases dramatically after birth and only trace amounts are expressed in the adult liver. High serum AFP concentrations are also found in connection with primary liver cancer and teratocarcinoma.