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验证数据展示

  • WB analysis of mouse skin using 83335-4-RR (same clone as 83335-4-PBS)

    WB analysis of mouse skin using 83335-4-RR (same clone as 83335-4-PBS)

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 83335-4-RR (IL-1RN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83335-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse skin using 83335-4-RR (same clone as 83335-4-PBS)

    IHC staining of mouse skin using 83335-4-RR (same clone as 83335-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 83335-4-RR (IL-1RN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83335-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse skin using 83335-4-RR (same clone as 83335-4-PBS)

    IHC staining of mouse skin using 83335-4-RR (same clone as 83335-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 83335-4-RR (IL-1RN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83335-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00353-2

    Cytometric bead array standard curve of MP00353-2

    Cytometric bead array standard curve of MP00353-2, MOUSE IL-1RA Recombinant Matched Antibody Pair - PBS only. Capture antibody: 83335-4-PBS. Detection antibody: 83335-3-PBS. Standard: Eg0685. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 83335-4-RR

    Affinity and Kinetic Characterization of 83335-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83335-4-RR against Mouse IL-1RN were performed. The affinity constant is 1.75 nM.

Mouse IL-1RA  Recombinant antibody, PBS Only (Capture)

IL-1RA  Uni-rAbTM Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 83335-4-PBS

产品说明书

CloneNo. 240208G5

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, Cytometric bead array, Indirect ELISA

别名

IL-1RN, Il1rn, Interleukin-1, IL-1RA, IL 1ra

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83335-4-PBS targets IL-1RA  as part of a matched antibody pair:

MP00353-2: 83335-4-PBS capture and 83335-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin 1 receptor antagonist
别名 IL-1RN, Il1rn, Interleukin-1, IL-1RA, IL 1ra
计算分子量18KD
观测分子量18 kDa
GenBank蛋白编号NM_031167.5
基因名称 IL-1RA 
Gene ID (NCBI) 16181
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ542W1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Interleukin-1 receptor antagonist (IL-1RA) is a critical member of the IL-1 family of cytokines, which plays a significant role in modulating inflammatory responses. IL-1RA exists in two main forms: a secreted form (sIL-1Ra) and an intracellular form (icIL-1Ra). The secreted form is produced by various cells including monocytes, macrophages, neutrophils, and other cells, while the intracellular form is found in keratinocytes and other epithelial cells, as well as in monocytes and fibroblasts. IL-1RA is important in host defense against endotoxin-induced injury and is produced in numerous experimental animal models of disease, as well as in human autoimmune and chronic inflammatory diseases. It acts as an anti-inflammatory agent by maintaining the balance between pro-inflammatory IL-1α/β and its own anti-inflammatory effects.