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验证数据展示

  • WB analysis of mouse skin using 83335-4-RR

    WB analysis of mouse skin using 83335-4-RR

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 83335-4-RR (IL-1RN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skin using 83335-4-RR

    IHC staining of mouse skin using 83335-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 83335-4-RR (IL-1RN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skin using 83335-4-RR

    IHC staining of mouse skin using 83335-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 83335-4-RR (IL-1RN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83335-4-RR

    Affinity and Kinetic Characterization of 83335-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83335-4-RR against Mouse IL-1RN were performed. The affinity constant is 1.75 nM.

IL-1RA  Recombinant antibody

IL-1RA  Recombinant Antibody for WB, IHC, ELISA
Cat No. 83335-4-RR

产品说明书

CloneNo. 240208G5

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, ELISA

别名

IL-1RN, Il1rn, Interleukin-1, IL-1RA, IL 1ra

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse skin tissue
Positive IHC detected inmouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83335-4-RR targets IL-1RA  in WB, IHC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin 1 receptor antagonist
别名 IL-1RN, Il1rn, Interleukin-1, IL-1RA, IL 1ra
计算分子量18KD
观测分子量18 kDa
GenBank蛋白编号NM_031167.5
基因名称 IL-1RA 
Gene ID (NCBI) 16181
RRIDAB_3670997
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ542W1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interleukin-1 receptor antagonist (IL-1RA) is a critical member of the IL-1 family of cytokines, which plays a significant role in modulating inflammatory responses. IL-1RA exists in two main forms: a secreted form (sIL-1Ra) and an intracellular form (icIL-1Ra). The secreted form is produced by various cells including monocytes, macrophages, neutrophils, and other cells, while the intracellular form is found in keratinocytes and other epithelial cells, as well as in monocytes and fibroblasts. IL-1RA is important in host defense against endotoxin-induced injury and is produced in numerous experimental animal models of disease, as well as in human autoimmune and chronic inflammatory diseases. It acts as an anti-inflammatory agent by maintaining the balance between pro-inflammatory IL-1α/β and its own anti-inflammatory effects.

实验方案

Product Specific Protocols
WB protocol for IL-1RA  antibody 83335-4-RRDownload protocol
IHC protocol for IL-1RA  antibody 83335-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols