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验证数据展示

  • WB analysis of HEK-293 using 83204-2-RR (same clone as 83204-2-PBS)

    WB analysis of HEK-293 using 83204-2-RR (same clone as 83204-2-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 83204-2-RR (HAS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83204-2-PBS in a different storage buffer formulation.

  • WB analysis using 83204-2-RR (same clone as 83204-2-PBS)

    WB analysis using 83204-2-RR (same clone as 83204-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83204-2-RR (HAS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83204-2-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 83204-2-RR (same clone as 83204-2-PBS)

    WB analysis of HEK-293 using 83204-2-RR (same clone as 83204-2-PBS)

    WB result of HAS2 antibody (83204-2-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-HAS2 transfected HEK-293 cells. This data was developed using the same antibody clone with 83204-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00153-1

    Cytometric bead array standard curve of MP00153-1

    Cytometric bead array standard curve of MP00153-1, HAS2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83204-2-PBS. Detection antibody: 83204-1-PBS. Standard: Ag34945. Range: 0.625-20 ng/mL.

  • Cytometric bead array standard curve of MP00153-3

    Cytometric bead array standard curve of MP00153-3

    Cytometric bead array standard curve of MP00153-3, HAS2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83204-2-PBS. Detection antibody: 83204-3-PBS. Standard: Ag34945. Range: 0.625-20 ng/mL.

  • Affinity and Kinetic Characterization of 83204-2-PBS

    Affinity and Kinetic Characterization of 83204-2-PBS

    Biolayer interferometry (BLl) kinetic assay of 83204-2-PBS against Human HAS2 was performed. The affinity constant is 0.516 nM.

  • Affinity and Kinetic Characterization of 83204-2-RR

    Affinity and Kinetic Characterization of 83204-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83204-2-RR against Human HAS2 were performed. The affinity constant is 1.37 nM.

  • Featured Product
  • KD/KO验证

HAS2 Recombinant monoclonal antibody, PBS Only (Capture)

HAS2 Uni-rAb® Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 83204-2-PBS

产品说明书

CloneNo. 240061G4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, Cytometric bead array, Indirect ELISA

别名

240061G4, EC:2.4.1.212, HA synthase 2, hyaluronan synthase 2, Hyaluronate synthase 2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83204-2-PBS targets HAS2 as part of a matched antibody pair:

MP00153-1: 83204-2-PBS capture and 83204-1-PBS detection (validated in Cytometric bead array)

MP00153-3: 83204-2-PBS capture and 83204-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag34945

Product name: Recombinant human HAS2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 67-170 aa of BC069353

Sequence: EHRKMKKSLETPIKLNKTVALCIAAYQEDPDYLRKCLQSVKRLTYPGIKVVMVIDGNSEDDLYMMDIFSEVMGRDKSATYIWKNNFHEKGPGETDESHKESSQH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 hyaluronan synthase 2
别名 240061G4, EC:2.4.1.212, HA synthase 2, hyaluronan synthase 2, Hyaluronate synthase 2
计算分子量 552 aa, 64 kDa
观测分子量 63-67 kDa
GenBank蛋白编号BC069353
基因名称 HAS2
Gene ID (NCBI) 3037
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ92819
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RIT2 (Hyaluronan synthase 2) is a member of the gene family encoding the hyaluronan synthase 2, which can generate high-molecular-weight hyaluronan (HMW-HA) (PMID: 35069543). HAS2 is the most critical synthase in producing hyaluronan and is well known for its involvement in cancer growth, metabolism and metastasis (PMID: 36386154). HAS2 is involved in cellular acquired resistance to drug therapy in BrCa. HAS2 expression was decreased in the endocrine-resistant ER+BrCa cells.

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