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验证数据展示

  • IF Staining of MCF-7 using 83188-2-RR

    IF Staining of MCF-7 using 83188-2-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using MMP13 antibody (83188-2-RR, Clone: 240060B10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of MCF-7 using 83188-2-RR

    FC experiment of MCF-7 using 83188-2-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug MMP13 Recombinant antibody (83188-2-RR, Clone:240060B10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 83188-2-RR

    Affinity and Kinetic Characterization of 83188-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83188-2-RR against Human MMP13 were performed. The affinity constant is below 1 pM.

MMP13 Recombinant monoclonal antibody

MMP13 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83188-2-RR

产品说明书

CloneNo. 240060B10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

240060B10, CLG3, Collagenase 3, EC:3.4.24.-, Matrix metalloproteinase 13

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83188-2-RR targets MMP13 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag34892

Product name: Recombinant human MMP13 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 100-471 aa of BC074808

Sequence: DVGEYNVFPRTLKWSKMNLTYRIVNYTPDMTHSEVEKAFKKAFKVWSDVTPLNFTRLHDGIADIMISFGIKEHGDFYPFDGPSGLLAHAFPPGPNYGGDAHFDDDETWTSSSKGYNLFLVAAHEFGHSLGLDHSKDPGALMFPIYTYTGKSHFMLPDDDVQGIQSLYGPGDEDPNPKHPKTPDKCDPSLSLDAITSLRGETMIFKDRFFWRLHPQQVDAELFLTKSFWPELPNRIDAAYEHPSHDLIFIFRGRKFWALNGYDILEGYPKKISELGLPKEVKKISAAVHFEDTGKTLLFSGNQVWRYDDTNHIMDKDYPRLIEEDFPGIGDKVDAVYEKNGYIYFFNGPIQFEYSIWSNRIVRVMPANSILWC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 matrix metallopeptidase 13
别名 240060B10, CLG3, Collagenase 3, EC:3.4.24.-, Matrix metalloproteinase 13
计算分子量 471 aa, 54 kDa
观测分子量 70 kDa, 54 kDa
GenBank蛋白编号BC074808
基因名称 MMP13
Gene ID (NCBI) 4322
RRIDAB_3670877
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP45452
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MMP-13(Matrix metalloproteinase-13), cleaves type I collagen and was previously thought to be the rodent analogue of human interstitial MMP-1. MMP-13 is found in hypertrophic and calcifying cartilage of mammalian growth plate. The bands seen with rat MMP-13 are consistent with a 59-kDa latent form and a 43-kDa active form reported for this enzyme, along with a smaller band at approximately 30 kDa(PMID:10771523).

实验方案

Product Specific Protocols
FC protocol for MMP13 antibody 83188-2-RRDownload protocol
IF protocol for MMP13 antibody 83188-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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