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验证数据展示

  • WB analysis using 83092-1-RR

    WB analysis using 83092-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83092-1-RR (NRF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 83092-1-RR

    IF Staining of HepG2 using 83092-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NRF1/nuclear respiratory factor 1 antibody (83092-1-RR, Clone: 230394B1 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • WB analysis of HeLa using 83092-1-RR

    WB analysis of HeLa using 83092-1-RR

    WB result of NRF1/nuclear respiratory factor 1 antibody (83092-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NRF1/nuclear respiratory factor 1 transfected HeLa cells.

  • IHC staining of human cervical squamous cancer using 83092-1-RR

    IHC staining of human cervical squamous cancer using 83092-1-RR

    Immunohistochemical analysis of paraffin-embedded human cervical squamous cancer tissue slide using 83092-1-RR (NRF1/nuclear respiratory factor 1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NRF1 antibody included
  • FC experiment of HEK-293T using 83092-1-RR

    FC experiment of HEK-293T using 83092-1-RR

    1x10^6 HEK-293T cells were intracellularly stained with 0.25 ug Anti-Human NRF1 (83092-1-RR, Clone:230394B1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83092-1-RR

    Affinity and Kinetic Characterization of 83092-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83092-1-RR against Human NRF1/nuclear respiratory factor 1 were performed. The affinity constant is 0.115 nM.

  • Featured Product
  • KD/KO验证

NRF1/nuclear respiratory factor 1 Recombinant monoclonal antibody

NRF1/nuclear respiratory factor 1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83092-1-RR

产品说明书

CloneNo. 230394B1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

NRF1, nuclear respiratory factor 1, 230394B1, ALPHA PAL, Alpha palindromic-binding protein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, RAW 264.7 cells, NIH/3T3 cells, U2OS cells
Positive IHC detected inhuman cervical squamous cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHEK-293T cells
Planning an IHC experiment? We recommend our IHCeasy NRF1 Ready-To-Use IHC Kit. NRF1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83092-1-RR targets NRF1/nuclear respiratory factor 1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag3172

Product name: Recombinant human NRF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 213-522 aa of BC016925

Sequence: LKYSTGRGKPGWGKESCKPIWWPEDIPWANVRSDVRTEEQKQRVSWTQALRTIVKNCYKQHGREDLLYAFEDQQTQTQATATHSIAHLVPSQTVVQTFSNPDGTVSLIQVGTGATVATLADASELPTTVTVAQVNYSAVADGEVEQNWATLQGGEMTIQTTQASEATQAVASLAEAAVAASQEMQQGATVTMALNSEAAAHAVATLAEATLQGGGQIVLSGETAAAVGALTGVQDANGLFMADRAGRKWILTDKATGLVQIPVSMYQTVVTSLAQGNGPVQVAMAPVTTRISDSAVTMDGQAVEVVTLEQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nuclear respiratory factor 1
别名 NRF1, nuclear respiratory factor 1, 230394B1, ALPHA PAL, Alpha palindromic-binding protein
计算分子量 522 aa, 56 kDa
观测分子量 70 kDa
GenBank蛋白编号BC016925
基因名称 NRF1
Gene ID (NCBI) 4899
RRIDAB_3670806
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ16656
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NRF1, also named as Alpha-pal and NRF-1, belongs to the NRF1/Ewg family. It is a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. NRF1 is implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. It plays a critical role in regulating the expression of many antioxidant response element (ARE)-dependent genes. NRF1 exists various isoforms and long isoform is about 67 kDa and short isoform is about 45 kDa.

实验方案

Product Specific Protocols
IF protocol for NRF1/nuclear respiratory factor 1 antibody 83092-1-RRDownload protocol
IHC protocol for NRF1/nuclear respiratory factor 1 antibody 83092-1-RRDownload protocol
WB protocol for NRF1/nuclear respiratory factor 1 antibody 83092-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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