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验证数据展示

  • FC experiment of THP-1 using 83078-4-RR (same clone as 83078-4-PBS)

    FC experiment of THP-1 using 83078-4-RR (same clone as 83078-4-PBS)

    1x10^6 THP-1 cells were intracellularly stained with 0.25 ug p300 Recombinant antibody (83078-4-RR, Clone:230369H8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83078-4-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83078-4-RR (same clone as 83078-4-PBS)

    IF Staining of HepG2 using 83078-4-RR (same clone as 83078-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using p300 antibody (83078-4-RR, Clone: 230369H8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83078-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00310-2

    Cytometric bead array standard curve of MP00310-2

    Cytometric bead array standard curve of MP00310-2, p300 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 83078-4-PBS. Detection antibody: 83078-3-PBS. Standard: SY00911. Range: 0.313-5 ng/mL.

p300 Recombinant antibody, PBS Only (Capture)

p300 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83078-4-PBS

产品说明书

CloneNo. 230369H8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

EP300, Histone butyryltransferase p300, EC:2.3.1.48, EC:2.3.1.-, E1A-associated protein p300

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy EP300 Ready-To-Use IHC Kit. EP300 primary antibody included.

产品信息

83078-4-PBS targets p300 as part of a matched antibody pair:

MP00310-2: 83078-4-PBS capture and 83078-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
亲和力
KD=1.30 x 10-8M
KOff=3.14 x 10-3M
KOn=2.42 x 105M
全称 E1A binding protein p300
别名 EP300, Histone butyryltransferase p300, EC:2.3.1.48, EC:2.3.1.-, E1A-associated protein p300
计算分子量 264 kDa
GenBank蛋白编号NM_001429
基因名称 EP300
Gene ID (NCBI) 2033
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ09472
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C