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验证数据展示

  • ChIP experiment of NCCIT using 82958-6-RR

    ChIP experiment of NCCIT using 82958-6-RR

    Chromatin was prepared from NCCIT cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of MAX (82958-6-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

  • WB analysis using 82958-6-RR

    WB analysis using 82958-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82958-6-RR (MAX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of PC-3 using 82958-6-RR

    IF Staining of PC-3 using 82958-6-RR

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using MAX antibody (82958-6-RR, Clone: 230215D9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 82958-6-RR

    Affinity and Kinetic Characterization of 82958-6-RR

    Biolayer interferometry (BLl) kinetic assays of 82958-6-RR against Human MAX were performed. The affinity constant is 0.257 nM.

MAX Recombinant monoclonal antibody

MAX Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA, ChIP-qPCR
Cat No. 82958-6-RR

产品说明书

CloneNo. 230215D9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA, ChIP-qPCR

别名

bHLHd4, bHLHd5, bHLHd6, bHLHd7, bHLHd8

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inPC-3 cells, HEK-293 cells, HL-60 cells, A431 cells, Ramos cells, NIH/3T3 cells, C6 cells
Positive IF/ICC detected inPC-3 cells
Positive ChIP-qPCR detected inNCCIT cells
Planning an IHC experiment? We recommend our IHCeasy MAX Ready-To-Use IHC Kit. MAX primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
CHIP-QPCRCHIP-QPCR : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82958-6-RR targets MAX in WB, IF/ICC, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA, ChIP-qPCR Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0680

Product name: Recombinant human MAX protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-151 aa of BC003525

Sequence: MSDNDDIEVESDADKRAHHNALERKRRDHIKDSFHSLRDSVPSLQGEKASRAQILDKATEYIQYMRRKNHTHQQDIDDLKRQNALLEQQVRALEKARSSAQLQTNYPSSDNSLYTNAKGSTISAFDGGSDSSSESEPEEPQSRKKLRMEAS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 MYC associated factor X
别名 bHLHd4, bHLHd5, bHLHd6, bHLHd7, bHLHd8
计算分子量 18 kDa
观测分子量22 kDa
GenBank蛋白编号BC003525
基因名称 MAX
Gene ID (NCBI) 4149
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP61244
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Max, a member of MAX family, contains a basic helix-loop-helix (bHLH) domain, is a transcription regulator. MAX can form a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC-MAX complex is a transcriptional activator, while the MAD-MAX complex is a repressor. MAX could dimerizated with another bHLH protein to form a heterodimer, such MYC or MAD. Thus bands recognized by this antibody much larger than predicted.

实验方案

Product Specific Protocols
IF protocol for MAX antibody 82958-6-RRDownload protocol
WB protocol for MAX antibody 82958-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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