验证数据展示
经过测试的应用
| Positive WB detected in | THP-1 cells, mouse liver tissue, NIH/3T3 cells, HSC-T6 cells |
| Positive Dot Blot detected in | peptide |
This antibody specifically recognize symmetric dimethyl-arginine (SDMA, Rme2s).
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| DOT BLOT | DOT BLOT : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82859-1-RR targets Di-Methyl-Histone H4 (Arg3) in WB, Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, Dot Blot, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | histone cluster 1, H4a |
| 别名 | Di-Methyl-Histone H4, Di-Methyl Histone-H4 Arg3, Di-Methyl Histone H4 Arg3, Di-Methyl Histone H4 (Arg3), Di Methyl Histone H4 |
| 观测分子量 | 13 kDa |
| GenBank蛋白编号 | BC069654 |
| 基因名称 | HIST1H4A |
| Gene ID (NCBI) | 8359 |
| RRID | AB_3670583 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P62805 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Di-Methyl-Histone H4 (Arg3) antibody 82859-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
