验证数据展示
经过测试的应用
| Positive WB detected in | THP-1 cells, mouse liver tissue, NIH/3T3 cells, HSC-T6 cells |
| Positive Dot Blot detected in | peptide |
This antibody specifically recognize symmetric dimethyl-arginine (SDMA, Rme2s).
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| DOT BLOT | DOT BLOT : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82859-1-RR targets Di-Methyl-Histone H4 (Arg3) in WB, Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, Dot Blot, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | histone cluster 1, H4a |
| 别名 | Di Methyl Histone H4, Di-Methyl Histone H4 (Arg3), Di-Methyl Histone H4 Arg3, Di-Methyl Histone-H4 Arg3, Di-Methyl-Histone H4 |
| 观测分子量 | 13 kDa |
| GenBank蛋白编号 | BC069654 |
| 基因名称 | HIST1H4A |
| Gene ID (NCBI) | 8359 |
| RRID | AB_3670583 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P62805 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Arginine dimethylation is an important post-translational modification of proteins. Based on the positions of the two methyl groups on the guanidino group of arginine, it can be divided into symmetric dimethylation (SDMA) and asymmetric dimethylation (ADMA). Symmetric dimethylation is crucial for embryonic development and cell differentiation and is often associated with transcriptional repression. This antibody specifically recognizes symmetric dimethyl-arginine (SDMA, Rme2s).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Di-Methyl-Histone H4 (Arg3) antibody 82859-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
