验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, A549 cells, mouse brain tissue, rat brain tissue, pig liver tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
81615-1-RR targets pan-RAS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 经测试反应性 | Human, mouse, rat, pig |
| 免疫原 | pan-RAS fusion protein Ag2700 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog |
| 别名 | KRAS, KRAS4A, KRAS4B, pan RAS, EC:3.6.5.2 |
| 计算分子量 | 188 aa, 21 kDa |
| 观测分子量 | 21 kDa |
| GenBank蛋白编号 | BC013572 |
| 基因名称 | KRAS |
| Gene ID (NCBI) | 3845 |
| RRID | AB_2935567 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P01116 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) belong to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. K-Ras, H-Ras, and N-Ras have similar structure and sequences. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. The ras genes are ubiquitously expressed although mRNA analysis suggests different level expression in tissue. Mutations in each ras gene frequently were found in different tumors, suggesting their involvement in the development of specific neoplasia. This antibody can recognize K-Ras, H-Ras, and N-Ras.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for pan-RAS antibody 81615-1-RR | Download protocol |
| IHC protocol for pan-RAS antibody 81615-1-RR | Download protocol |
| IF protocol for pan-RAS antibody 81615-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |