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验证数据展示

  • WB analysis using 68516-1-Ig

    WB analysis using 68516-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68516-1-Ig (ATL3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 68516-1-Ig

    WB analysis of Jurkat using 68516-1-Ig

    WB result of ATL3 antibody (68516-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATL3 transfected Jurkat cells.

  • IF Staining of HeLa using 68516-1-Ig

    IF Staining of HeLa using 68516-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATL3 antibody (68516-1-Ig, Clone: 2E7C4 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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  • KD/KO验证

ATL3 Monoclonal antibody

ATL3 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68516-1-Ig

产品说明书

CloneNo. 2E7C4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, rabbit

应用

WB, IF/ICC, ELISA

别名

EC:3.6.5.-, Atlastin-3, Atlastin 3, 2E7C4

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inPC-3 cells, Jurkat cells, HeLa cells, K-562 cells, rabbit testis tissue, rat testis tissue, mouse testis tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68516-1-Ig targets ATL3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat, rabbit
免疫原

CatNo: Ag10248

Product name: Recombinant human ATL3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-351 aa of BC077727

Sequence: MLSPQRVAAAASRGADDAMESSKPGPVQVVLVQKDQHSFELDEKALASILLQDHIRDLDVVVVSVAGAFRKGKSFILDFMLRYLYSQKESGHSNWLGDPEEPLTGFSWRGGSDPETTGIQIWSEVFTVEKPGGKKVAVVLMDTQGAFDSQSTVKDCATIFALSTMTSSVQIYNLSQNIQEDDLQQLQLFTEYGRLAMDEIFQKPFQTLMFLVRDWSFPYEYSYGLQGGMAFLDKRLQVKEHQHEEIQNVRNHIHSCFSDVTCFLLPHPGLQVATSPDFDGKLKDIAGEFKEQLQALIPYVLNPSKLMEKEINGSKVTCRGLLEYFKAYIKIYQGEDLPHPKSMLQATAEAN

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 atlastin GTPase 3
别名 EC:3.6.5.-, Atlastin-3, Atlastin 3, 2E7C4
计算分子量 541 aa, 61 kDa
观测分子量 61 kDa
GenBank蛋白编号BC077727
基因名称 ATL3
Gene ID (NCBI) 25923
RRIDAB_3085225
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ6DD88
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATL3, belonging to a class of dynamin-like GTPase, was reported to mediate ER fusion. ATL3 is required for the proper formation of the network of interconnected tubules of the endoplasmic reticulum. ATL3 functions as a receptor for ER-phagy, promoting tubular ER degradation upon starvation. Mutations in this gene may be associated with hereditary sensory neuropathy type IF.

实验方案

Product Specific Protocols
IF protocol for ATL3 antibody 68516-1-IgDownload protocol
WB protocol for ATL3 antibody 68516-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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