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验证数据展示

  • WB analysis using 68511-1-Ig (same clone as 68511-1-PBS)

    WB analysis using 68511-1-Ig (same clone as 68511-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68511-1-Ig (POLR2F antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68511-1-PBS in a different storage buffer formulation.

  • WB analysis using 68511-1-Ig (same clone as 68511-1-PBS)

    WB analysis using 68511-1-Ig (same clone as 68511-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68511-1-Ig (POLR2F antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68511-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51851-1

    Cytometric bead array sample test of MP51851-1

    Cytometric bead array sample test of MP51851-1, POLR2F Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68511-2-PBS. Detection antibody: 68511-1-PBS.

  • Cytometric bead array standard curve of MP51851-1

    Cytometric bead array standard curve of MP51851-1

    Cytometric bead array standard curve of MP51851-1, POLR2F Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68511-2-PBS. Detection antibody: 68511-1-PBS. Standard:Ag7472. Range: 0.781-100 ng/mL.

POLR2F Monoclonal antibody, PBS Only (Detector)

POLR2F Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 68511-1-PBS

产品说明书

CloneNo. 2D4D12

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse

应用

WB, Cytometric bead array, Indirect ELISA

别名

2D4D12, DNA-directed RNA polymerase II subunit F, DNA-directed RNA polymerases I, II, and III 14.4 kDa polypeptide, DNA-directed RNA polymerases I, II, and III subunit RPABC2, HRBP14.4

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy POLR2F Ready-To-Use IHC Kit. POLR2F primary antibody included.

产品信息

68511-1-PBS targets POLR2F as part of a matched antibody pair:

MP51851-1: 68511-2-PBS capture and 68511-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag7472

Product name: Recombinant human POLR2F protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-127 aa of BC003582

Sequence: MSDNEDNFDGDDFDDVEEDEGLDDLENAEEEGQENVEILPSGERPQANQKRITTPYMTKYERARVLGTRALQIAMCAPVMVELEGETDPLLIAMKELKARKIPIIIRRYLPDGSYEDWGVDELIITD

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 polymerase (RNA) II (DNA directed) polypeptide F
别名 2D4D12, DNA-directed RNA polymerase II subunit F, DNA-directed RNA polymerases I, II, and III 14.4 kDa polypeptide, DNA-directed RNA polymerases I, II, and III subunit RPABC2, HRBP14.4
计算分子量 14 kDa
观测分子量 23 kDa
GenBank蛋白编号BC003582
基因名称 POLR2F
Gene ID (NCBI) 5435
RRIDAB_3085220
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP61218
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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