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验证数据展示

  • WB analysis using 68454-1-Ig

    WB analysis using 68454-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68454-1-Ig (DDX43 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68454-1-Ig

    WB analysis using 68454-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68454-1-Ig (DDX43 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68454-1-Ig

    WB analysis using 68454-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68454-1-Ig (DDX43 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

DDX43 Monoclonal antibody

DDX43 Monoclonal Antibody for WB, ELISA
Cat No. 68454-1-Ig

产品说明书

CloneNo. 1B3B2

宿主/亚型

Mouse / IgG1

种属反应性

Human, Rat, Rabbit

应用

WB, ELISA

别名

Cancer/testis antigen 13, CT13, DDX43, DEAD box protein 43, DEAD box protein HAGE, DKFZp434H2114, HAGE, Helical antigen

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inCalu-3 cells, LNCaP cells, HeLa cells, U2OS cells, DU 145 cells, NCCIT cells, rabbit testis tissue, rat testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68454-1-Ig targets DDX43 in WB, ELISA applications and shows reactivity with Human, Rat, Rabbit samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, Rat, Rabbit
免疫原

CatNo: Ag11484

Product name: Recombinant human DDX43 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 264-648 aa of BC066938

Sequence: KPTPIQSQAWPIVLQGIDLIGVAQTGTGKTLCYLMPGFIHLVLQPSLKGQRNRPGMLVLTPTRELALQVEGECCKYSYKGLRSVCVYGGGNRDEQIEELKKGVDIIIATPGRLNDLQMSNFVNLKNITYLVLDEADKMLDMGFEPQIMKILLDVRPDRQTVMTSATWPHSVHRLAQSYLKEPMIVYVGTLDLVAVSSVKQNIIVTTEEEKWSHMQTFLQSMSSTDKVIVFVSRKAVADHLSSDLILGNISVESLHGDREQRDREKALENFKTGKVRILIATDLASRGLDVHDVTHVYNFDFPRNIEEYVHRIGRTGRAGRTGVSITTLTRNDWRVASELINILERANQSIPEELVSMAERFKAHQQKREMERKMERPQGRPKKFH

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-Asp) box polypeptide 43
别名 Cancer/testis antigen 13, CT13, DDX43, DEAD box protein 43, DEAD box protein HAGE, DKFZp434H2114, HAGE, Helical antigen
计算分子量 648 aa, 73 kDa
观测分子量 68-73 kDa
GenBank蛋白编号BC066938
基因名称 DDX43
Gene ID (NCBI) 55510
RRIDAB_3085166
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9NXZ2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDX43 (DEAD-box helicase 43), also known as HAGE (helicase antigen gene), is a member of the DEAD-box protein family. DDX43 is highly expressed in many tumors and is, therefore, considered a potential target for immunotherapy.

实验方案

Product Specific Protocols
WB protocol for DDX43 antibody 68454-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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