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验证数据展示

  • WB analysis using 68403-1-Ig

    WB analysis using 68403-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68403-1-Ig (HIP1R antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of 4T1 using 68403-1-Ig

    WB analysis of 4T1 using 68403-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 68403-1-Ig (HIP1R antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 68403-1-Ig

    IF Staining of HeLa using 68403-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HIP1R antibody (68403-1-Ig, Clone: 2F10D10 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

HIP1R Monoclonal antibody

HIP1R Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68403-1-Ig

产品说明书

CloneNo. 2F10D10

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

FLJ14000, FLJ27022, HIP 12, HIP1 related protein, HIP12, HIP1R, HIP3, ILWEQ, KIAA0655

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, 4T1 cells, U2OS cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68403-1-Ig targets HIP1R in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原

CatNo: Ag10657

Product name: Recombinant human HIP1R protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 266-615 aa of BC067085

Sequence: RASDMLYFKRLIQIPRLPEGPPNFLRASALAEHIKPVVVIPEEAPEDEEPENLIEISTGPPAGEPVVVADLFDQTFGPPSGSVKDDRDLQIESLKREVEMLRSELEKIKLEAQRYIAQLKSQVNALEGELEEQRKQKQKALVDNEQLRHELAQLRAAQLEGERSQGLREEAERKASATEARYNKLKEKHSELVHVHAELLRKNADTAKQLTVTQQSQEEVARVKEQLAFQVEQVKRESELKLEEKSDQLEKLKRELEAKAGELARAQEALSHTEQSKSELSSRLDTLSAEKDALSGAVRQREADLLAAQSLVRETEAALSREQQRSSQEQGELQGRLAERVWPPQMQQHH

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 huntingtin interacting protein 1 related
别名 FLJ14000, FLJ27022, HIP 12, HIP1 related protein, HIP12, HIP1R, HIP3, ILWEQ, KIAA0655
计算分子量 615 aa, 70 kDa, 120 kDa
观测分子量 120 kDa
GenBank蛋白编号BC067085
基因名称 HIP1R
Gene ID (NCBI) 9026
RRIDAB_3085120
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO75146
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HIP1R antibody 68403-1-IgDownload protocol
IF protocol for HIP1R antibody 68403-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols