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验证数据展示

  • WB analysis using 68279-1-Ig

    WB analysis using 68279-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68279-1-Ig (BDH2 antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • WB analysis of HeLa using 68279-1-Ig

    WB analysis of HeLa using 68279-1-Ig

    WB result of BDH2 antibody (68279-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BDH2 transfected HeLa cells.

  • IF Staining of HepG2 using 68279-1-Ig

    IF Staining of HepG2 using 68279-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BDH2 antibody (68279-1-Ig, Clone: 1B6H5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  • KD/KO验证

BDH2 Monoclonal antibody

BDH2 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68279-1-Ig

产品说明书

CloneNo. 1B6H5

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

(R)-beta-hydroxybutyrate dehydrogenase, 1B6H5, 3-hydroxybutyrate dehydrogenase type 2, 4-oxo-L-proline reductase, DHRS6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inBxPC-3 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68279-1-Ig targets BDH2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag26108

Product name: Recombinant human BDH2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-245 aa of BC095414

Sequence: MGRLDGKVIILTAAAQGIGQAAALAFAREGAKVIATDINESKLQELEKYPGIQTRVLDVTKKKQIDQFANEVERLDVLFNVAGFVHHGTVLDCEEKDWDFSMNLNVRSMYLMIKAFLPKMLAQKSGNIINMSSVASSVKGVVNRCVYSTTKAAVIGLTKSVAADFIQQGIRCNCVCPGTVDTPSLQERIQARGNPEEARNDFLKRQKTGRFATAEEIAMLCVYLASDESTYVTGNPVIIDGGWSL

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 3-hydroxybutyrate dehydrogenase, type 2
别名 (R)-beta-hydroxybutyrate dehydrogenase, 1B6H5, 3-hydroxybutyrate dehydrogenase type 2, 4-oxo-L-proline reductase, DHRS6
观测分子量 27 kDa
GenBank蛋白编号BC095414
基因名称 BDH2
Gene ID (NCBI) 56898
RRIDAB_2935360
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9BUT1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cytosolic type 2-hydroxybutyrate dehydrogenase, BDH2, originally named DHRS6, plays a physiological role in the cytosolic utilization of ketone bodies, which can subsequently enter mitochondria and the tricarboxylic acid cycle (PMID: 16380372). Moreover, BDH2 catalyzes the production of 2, 3-DHBA during enterobactin biosynthesis and participates in 24p3 (LCN2)-mediated iron transport and apoptosis (PMID: 23150981). It has 3 isoforms with molecular weights of 27, 19, and 16 kDa.

实验方案

Product Specific Protocols
WB protocol for BDH2 antibody 68279-1-IgDownload protocol
IF protocol for BDH2 antibody 68279-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols