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验证数据展示

  • WB analysis using 68148-1-Ig

    WB analysis using 68148-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68148-1-Ig (EEF1G antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68148-1-Ig

    WB analysis using 68148-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68148-1-Ig (EEF1G antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of MCF-7 using 68148-1-Ig

    WB analysis of MCF-7 using 68148-1-Ig

    WB result of EEF1G antibody (68148-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF1G transfected MCF-7 cells.

  • IF Staining of HeLa using 68148-1-Ig

    IF Staining of HeLa using 68148-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EEF1G antibody (68148-1-Ig, Clone: 1G7C6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of HeLa using 68148-1-Ig

    IF Staining of HeLa using 68148-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EEF1G antibody (68148-1-Ig, Clone: 1G7C6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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EEF1G Monoclonal antibody

EEF1G Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68148-1-Ig

产品说明书

CloneNo. 1G7C6

宿主/亚型

Mouse / IgG2b

种属反应性

human, pig, rabbit

应用

WB, IF/ICC, ELISA

别名

eEF 1B gamma, eEF-1B gamma, EF 1 gamma, EF1G, EF-1-gamma

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inU2OS cells, HeLa cells, MCF-7 cells, pig heart tissue, A549 cells, HEK-293 cells, rabbit heart tissue, pig brain tissue, rabbit brain tissue
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68148-1-Ig targets EEF1G in WB, IF/ICC, ELISA applications and shows reactivity with human, pig, rabbit samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, pig, rabbit
免疫原

CatNo: Ag0218

Product name: Recombinant human EEF1G protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 140-400 aa of BC006520

Sequence: GLLDAYLKTRTFLVGERVTLADITVVCTLLWLYKQVLEPSFRQAFPNTNRWFLTCINQPQFRAVLGEVKLCEKMAQFDAKKFAETQPKKDTPRKEKGSREEKQKPQAERKEEKKAAAPAPEEEMDECEQALAAEPKAKDPFAHLPKSTFVLDEFKRKYSNEDTLSVALPYFWEHFDKDGWSLWYSEYRFPEELTQTFMSCNLITGMFQRLDKLRKNAFASVILFGTNNSSSISGVWVFRGQELAFPLSPDWQVDYESYTWR

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 eukaryotic translation elongation factor 1 gamma
别名 eEF 1B gamma, eEF-1B gamma, EF 1 gamma, EF1G, EF-1-gamma
计算分子量 50 kDa
观测分子量 50 kDa
GenBank蛋白编号BC006520
基因名称 EEF1G
Gene ID (NCBI) 1937
RRIDAB_2923672
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP26641
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Eukaryotic translation elongation factor 1 (EEF1G, synonym: EF1G) is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases.

实验方案

Product Specific Protocols
WB protocol for EEF1G antibody 68148-1-IgDownload protocol
IF protocol for EEF1G antibody 68148-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols