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验证数据展示

  • WB analysis using 68127-1-Ig (same clone as 68127-1-PBS)

    WB analysis using 68127-1-Ig (same clone as 68127-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68127-1-Ig (PIN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68127-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51574-1

    Cytometric bead array sample test of MP51574-1

    Cytometric bead array sample test of MP51574-1, PIN1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68127-2-PBS. Detection antibody: 68127-1-PBS.

  • IP experiment of HepG2 using 68127-1-Ig (same clone as 68127-1-PBS)

    IP experiment of HepG2 using 68127-1-Ig (same clone as 68127-1-PBS)

    IP result of anti-PIN1 (IP:68127-1-Ig, 4ug; Detection:68127-1-Ig 1:2000) with HepG2 cells lysate 1920 ug. This data was developed using the same antibody clone with 68127-1-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 68127-1-Ig (same clone as 68127-1-PBS)

    IF Staining of NIH/3T3 using 68127-1-Ig (same clone as 68127-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using PIN1 antibody (68127-1-Ig, Clone: 1F4G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68127-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51574-1

    Cytometric bead array standard curve of MP51574-1

    Cytometric bead array standard curve of MP51574-1, PIN1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68127-2-PBS. Detection antibody: 68127-1-PBS. Standard:Ag0767. Range: 1.563-200 ng/mL.

PIN1 Monoclonal antibody, PBS Only (Detector)

PIN1 Monoclonal Antibody for WB, IF/ICC, IP, Cytometric bead array, Indirect ELISA
Cat No. 68127-1-PBS

产品说明书

CloneNo. 1F4G3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IF/ICC, IP, Cytometric bead array, Indirect ELISA

别名

1F4G3, EC:5.2.1.8, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, Peptidyl-prolyl cis-trans isomerase Pin1, Pin 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PIN1 Ready-To-Use IHC Kit. PIN1 primary antibody included.

产品信息

68127-1-PBS targets PIN1 as part of a matched antibody pair:

MP51574-1: 68127-2-PBS capture and 68127-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, IP, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原

CatNo: Ag0767

Product name: Recombinant human PIN1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-163 aa of BC002899

Sequence: MADEEKLPPGWEKRMSRSSGRVYYFNHITNASQWERPSGNSSSGGKNGQGEPARVRCSHLLVKHSQSRRPSSWRQEKITRTKEEALELINGYIQKIKSGEEDFESLASQFSDCSSAKARGDLGAFSRGQMQKPFEDASFALRTGEMSGPVFTDSGIHIILRTE

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 peptidylprolyl cis/trans isomerase, NIMA-interacting 1
别名 1F4G3, EC:5.2.1.8, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, Peptidyl-prolyl cis-trans isomerase Pin1, Pin 1
计算分子量 18 kDa
观测分子量18 kDa
GenBank蛋白编号BC002899
基因名称 PIN1
Gene ID (NCBI) 5300
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ13526
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PIN1(Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1) is essential for mitosis progression in yeast cells and is hypothesized to perform the same role in mammalian cells. It might regulate cellular processes distinct from the cell cycle itself, such as terminal differentiation through a modulation of differentiation-specific gene expression(PMID:20801874). It colocalizes with NEK6 in the nucleus. Pin1 inhibition simultaneously blocks multiple cancer pathways, disrupts the desmoplastic and immunosuppressive TME, and upregulates PD-L1 and ENT1, rendering pancreatic ductal adenocarcinoma (PDAC) eradicable by immunochemotherapy (PMID: 34388391).

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