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验证数据展示

  • WB analysis using 68061-1-Ig

    WB analysis using 68061-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68061-1-Ig (NSF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of PC-12 using 68061-1-Ig

    WB analysis of PC-12 using 68061-1-Ig

    PC-12 cells were subjected to SDS PAGE followed by western blot with 68061-1-Ig (NSF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of SH-SY5Y using 68061-1-Ig

    WB analysis of SH-SY5Y using 68061-1-Ig

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 68061-1-Ig (NSF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 68061-1-Ig

    WB analysis of HEK-293 using 68061-1-Ig

    WB result of NSF antibody (68061-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSF transfected HEK-293 cells.

  • IF Staining of mouse brain using 68061-1-Ig

    IF Staining of mouse brain using 68061-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NSF antibody (68061-1-Ig, Clone: 2B6H6 ) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of rat brain using 68061-1-Ig

    IHC staining of rat brain using 68061-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68061-1-Ig (NSF antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

NSF Monoclonal antibody

NSF Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 68061-1-Ig

产品说明书

CloneNo. 2B6H6

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig, rabbit, chicken

应用

WB, IHC, IF-P, ELISA

别名

2B6H6, EC:3.6.4.6, NEM-sensitive fusion protein, N-ethylmaleimide-sensitive fusion protein, SKD2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inpig brain tissue, SH-SY5Y cells, PC-12 cells, HEK-293 cells, Rabbit brain tissue, Rat brain tissue, Mouse brain tissue, Chicken brain tissue
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)-PIF-P : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68061-1-Ig targets NSF in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit, chicken samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit, chicken
免疫原 NSF fusion protein Ag15422 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 N-ethylmaleimide-sensitive factor
别名 2B6H6, EC:3.6.4.6, NEM-sensitive fusion protein, N-ethylmaleimide-sensitive fusion protein, SKD2
计算分子量 744 aa, 83 kDa
观测分子量70-83 kDa
GenBank蛋白编号BC030613
基因名称 NSF
Gene ID (NCBI) 4905
RRIDAB_2918802
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP46459
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NSF antibody 68061-1-IgDownload protocol
IHC protocol for NSF antibody 68061-1-IgDownload protocol
IF protocol for NSF antibody 68061-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols