验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, HaCaT cells, Caco-2 cells, HT-29 cells, HeLa cells, NCCIT cells, JAR cells, ATDC-5 cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
67993-1-Ig targets SLC30A2 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | Human, Mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag8232 Product name: Recombinant human SLC30A2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 234-323 aa of BC006251 Sequence: KGVDFTAVRDLLLSVEGVEALHSLHIWALTVAQPVLSVHIAIAQNTDAQAVLKTASSRLQGKFHFHTVTIQIEDYSEDMKDCQACQGPSD 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 30 (zinc transporter), member 2 |
| 别名 | FLJ36708, PP12488, SLC30A2, Zinc transporter 2, ZnT 2, ZNT2 |
| 计算分子量 | 323 aa, 35 kDa |
| 观测分子量 | 41 kDa |
| GenBank蛋白编号 | BC006251 |
| 基因名称 | SLC30A2 |
| Gene ID (NCBI) | 7780 |
| RRID | AB_2918742 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q9BRI3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLC30A2, also named as the Zn transporter 2 gene (ZnT2), belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family and SLC30A subfamily. It plays a role in the zinc ion transmembrane transport. Expression of SLC30A2 is restricted to secretory cells, such as acinar pancreatic cells, prostate epithelial cells, placental trophoblasts, Paneth cells, and mammary epithelial cells (MECs). SLC30A2 consists of six transmembrane domains with cytoplasmic N- and C-termini that contain numerous regulatory domains, and functions as a homo- or hetero- dimer to transport Zn into vesicles (PMID: 29476070 ). SLC30A2 has 2 isoforms with the molecular mass of 35 and 41 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for SLC30A2 antibody 67993-1-Ig | Download protocol |
| WB protocol for SLC30A2 antibody 67993-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Adv Bimetallic peroxide nanoparticles induce PANoptosis by disrupting ion homeostasis for enhanced immunotherapy | ||
iScience Different populations of mouse egg cortical vesicles are responsible for post-fertilization zinc sparks and proteolysis of the zona pellucida |
