验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, PC-12 cells, RAW 264.7 cells |
| Positive IHC detected in | rat stomach tissue, human liver cancer tissue, mouse liver tissue, mouse stomach tissue, rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
67956-1-Ig targets Caspase 7 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | Human, mouse, rat |
| 免疫原 | Caspase 7 fusion protein Ag27601 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | caspase 7, apoptosis-related cysteine peptidase |
| 别名 | Apoptotic protease Mch 3, CASP 7, CASP7, Caspase 7, Caspase7, CMH 1, ICE LAP3, ICE like apoptotic protease 3, MCH3 |
| 计算分子量 | 303 aa, 34 kDa |
| 观测分子量 | 35 kDa |
| GenBank蛋白编号 | BC015799 |
| 基因名称 | Caspase 7 |
| Gene ID (NCBI) | 840 |
| RRID | AB_2918707 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P55210 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Caspase 7(CASP7), like caspases 3 and 6, contains a short prodomain and, upon apoptotic induction, the 35 kDa proform is converted into a 32 kDa intermediate or preactive form which is further processed into two active subunits consisting of the p20 or large (18 kDa) subunit and the p10 or small (11 kDa) subunit and it is present in the brain, which is up-regulated and activated after traumatic injury(PMID:15953353). Caspase-7 is classified as a member of the subgroup of cysteine proteases most related to the Caenorhabditis elegans factor CED-3, which also includes caspase-3, -6, and -9(PMID:9426061). The protein is involved in the activation cascade of caspases responsible for apoptosis execution.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Caspase 7 antibody 67956-1-Ig | Download protocol |
| IHC protocol for Caspase 7 antibody 67956-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Aquat Toxicol Study on low concentration deltamethrin toxicity mediated by phospholipase D in Chinese mitten crab (Eriocheir sinensis) ovary | ||
Cell Mol Life Sci The pluripotent-to-totipotent state transition in mESCs activates the intrinsic apoptotic pathway through DUX-induced DNA replication stress |
