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验证数据展示

  • WB analysis using 67734-1-Ig (same clone as 67734-1-PBS)

    WB analysis using 67734-1-Ig (same clone as 67734-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67734-1-Ig (CTPS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67734-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 67734-1-Ig (same clone as 67734-1-PBS)

    IHC staining of human prostate cancer using 67734-1-Ig (same clone as 67734-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67734-1-Ig (CTPS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67734-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50097-1

    Sandwich ELISA standard curve of MP50097-1

    Sandwich ELISA standard curve of MP50097-1, CTPS2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67734-1-PBS. Detection antibody: 67734-2-PBS. Standard:Ag30258. Range: 0.195-50 ng/mL.

  • IF Staining of human prostate cancer using 67734-1-Ig (same clone as 67734-1-PBS)

    IF Staining of human prostate cancer using 67734-1-Ig (same clone as 67734-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using CTPS2 antibody (67734-1-Ig, Clone: 1A3B8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67734-1-PBS in a different storage buffer formulation.

  • IF Staining of PC-12 using 67734-1-Ig (same clone as 67734-1-PBS)

    IF Staining of PC-12 using 67734-1-Ig (same clone as 67734-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-12 cells using CTPS2 antibody (67734-1-Ig, Clone: 1A3B8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67734-1-PBS in a different storage buffer formulation.

CTPS2 Monoclonal antibody, PBS Only (Capture)

CTPS2 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Sandwich ELISA, Indirect ELISA
Cat No. 67734-1-PBS

产品说明书

CloneNo. 1A3B8

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, Sandwich ELISA, Indirect ELISA

别名

1A3B8, CTP synthase 2, CTP synthase II, CTP synthetase 2, EC:6.3.4.2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67734-1-PBS targets CTPS2 as part of a matched antibody pair:

MP50097-1: 67734-1-PBS capture and 67734-2-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, IF-P, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag30258

Product name: Recombinant human CTPS2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 30-373 aa of BC034986

Sequence: CGLRVTAIKIDPYINIDAGTFSPYEHGEVFVLNDGGEVDLDLGNYERFLDINLYKDNNITTGKIYQHVINKERRGDYLGKTVQVVPHITDAVQEWVMNQAKVPVDGNKEEPQICVIELGGTIGDIEGMPFVEAFRQFQFKAKRENFCNIHVSLVPQLSATGEQKTKPTQNSVRALRGLGLSPDLIVCRSSTPIEMAVKEKISMFCHVNPEQVICIHDVSSTYRVPVLLEEQSIVKYFKERLHLPIGDSASNLLFKWRNMADRYERLQKICSIALVGKYTKLRDCYASVFKALEHSALAINHKLNLMYIDSIDLEKITETEDPVKFHEAWQKLCKADGILVPGGF

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 CTP synthase II
别名 1A3B8, CTP synthase 2, CTP synthase II, CTP synthetase 2, EC:6.3.4.2
计算分子量 586 aa, 66 kDa
观测分子量66 kDa
GenBank蛋白编号BC034986
基因名称 CTPS2
Gene ID (NCBI) 56474
RRIDAB_2882918
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9NRF8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CTPS2 (CTP synthase 2) is also named as UTP--ammonia ligase 2 and belongs to the CTP synthase family.It catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen and constitutes the rate-limiting enzyme in the synthesis of cytosine nucleotides. This antibody specifically detects only CTPS2 and not also CTPS1.

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