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验证数据展示

  • WB analysis using 67734-1-Ig

    WB analysis using 67734-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67734-1-Ig (CTPS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • IHC staining of human prostate cancer using 67734-1-Ig

    IHC staining of human prostate cancer using 67734-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67734-1-Ig (CTPS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human prostate cancer using 67734-1-Ig

    IF Staining of human prostate cancer using 67734-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using CTPS2 antibody (67734-1-Ig, Clone: 1A3B8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of PC-12 using 67734-1-Ig

    IF Staining of PC-12 using 67734-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-12 cells using CTPS2 antibody (67734-1-Ig, Clone: 1A3B8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

CTPS2 Monoclonal antibody

CTPS2 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 67734-1-Ig

产品说明书

CloneNo. 1A3B8

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, ELISA

别名

1A3B8, CTP synthase 2, CTP synthase II, CTP synthetase 2, EC:6.3.4.2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inLNCaP cells, U2OS cells, HeLa cells, HEK-293 cells, HepG2 cells, K-562 cells, PC-12 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman prostate cancer tissue
Positive IF/ICC detected inPC-12 cells

This antibody specifically detects only CTPS2 and not also CTPS1.

Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67734-1-Ig targets CTPS2 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag30258

Product name: Recombinant human CTPS2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 30-373 aa of BC034986

Sequence: CGLRVTAIKIDPYINIDAGTFSPYEHGEVFVLNDGGEVDLDLGNYERFLDINLYKDNNITTGKIYQHVINKERRGDYLGKTVQVVPHITDAVQEWVMNQAKVPVDGNKEEPQICVIELGGTIGDIEGMPFVEAFRQFQFKAKRENFCNIHVSLVPQLSATGEQKTKPTQNSVRALRGLGLSPDLIVCRSSTPIEMAVKEKISMFCHVNPEQVICIHDVSSTYRVPVLLEEQSIVKYFKERLHLPIGDSASNLLFKWRNMADRYERLQKICSIALVGKYTKLRDCYASVFKALEHSALAINHKLNLMYIDSIDLEKITETEDPVKFHEAWQKLCKADGILVPGGF

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 CTP synthase II
别名 1A3B8, CTP synthase 2, CTP synthase II, CTP synthetase 2, EC:6.3.4.2
计算分子量 586 aa, 66 kDa
观测分子量66 kDa
GenBank蛋白编号BC034986
基因名称 CTPS2
Gene ID (NCBI) 56474
RRIDAB_2882918
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9NRF8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CTPS2 (CTP synthase 2) is also named as UTP--ammonia ligase 2 and belongs to the CTP synthase family.It catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen and constitutes the rate-limiting enzyme in the synthesis of cytosine nucleotides. This antibody specifically detects only CTPS2 and not also CTPS1.

实验方案

Product Specific Protocols
IF protocol for CTPS2 antibody 67734-1-IgDownload protocol
IHC protocol for CTPS2 antibody 67734-1-IgDownload protocol
WB protocol for CTPS2 antibody 67734-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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