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验证数据展示

  • IHC staining of rat pancreas using 67603-1-Ig

    IHC staining of rat pancreas using 67603-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 67603-1-Ig (PNLIPRP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat pancreas using 67603-1-Ig

    IHC staining of rat pancreas using 67603-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 67603-1-Ig (PNLIPRP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 67603-1-Ig

    WB analysis using 67603-1-Ig

    pig pancreas tissue were subjected to SDS PAGE followed by western blot with 67603-1-Ig (PNLIPRP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

PNLIPRP1 Monoclonal antibody

PNLIPRP1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67603-1-Ig

产品说明书

CloneNo. 1F11E6
引用文献(1)

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse, rat, pig

应用

WB, IHC, ELISA

别名

PL RP1, PLRP1, PNLIPRP1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inpig pancreas tissue, rat pancreas tissue, mouse pancreas tissue
Positive IHC detected inrat pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67603-1-Ig targets PNLIPRP1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 Human, mouse, rat, pig
免疫原

CatNo: Ag26350

Product name: Recombinant human PNLIPRP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 18-234 aa of BC025784

Sequence: KEVCYEDLGCFSDTEPWGGTAIRPLKILPWSPEKIGTRFLLYTNENPNNFQILLLSDPSTIEASNFQMDRKTRFIIHGFIDKGDESWVTDMCKKLFEVEEVNCICVDWKKGSQATYTQAANNVRVVGAQVAQMLDILLTEYSYPPSKVHLIGHSLGAHVAGEAGSKTPGLSRITGLDPVEASFESTPEEVRLDPSDADFVDVIHTDAAPLIPFLGFG

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 pancreatic lipase-related protein 1
别名 PL RP1, PLRP1, PNLIPRP1
计算分子量 467 aa, 52 kDa
观测分子量 51 kDa
GenBank蛋白编号BC025784
基因名称 PNLIPRP1
Gene ID (NCBI) 5407
RRIDAB_2882809
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP54315
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PNLIPRP1 antibody 67603-1-IgDownload protocol
IHC protocol for PNLIPRP1 antibody 67603-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Biology (Basel)

Genome-Wide Scans for Selection Signatures in Haimen Goats Reveal Candidate Genes Associated with Growth Traits

Authors - Zhen Zhang
View Article