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验证数据展示

  • WB analysis using 67573-1-Ig (same clone as 67573-1-PBS)

    WB analysis using 67573-1-Ig (same clone as 67573-1-PBS)

    Human adipose tissue were subjected to SDS PAGE followed by western blot with 67573-1-Ig (Vaspin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67573-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67573-1-Ig (same clone as 67573-1-PBS)

    IF Staining of HeLa using 67573-1-Ig (same clone as 67573-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Vaspin antibody (67573-1-Ig, Clone: 2A1B6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67573-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51421-1

    Cytometric bead array sample test of MP51421-1

    Cytometric bead array sample test of MP51421-1, SERPINA12 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67573-1-PBS. Detection antibody: 67573-2-PBS.

  • Cytometric bead array standard curve of MP51421-1

    Cytometric bead array standard curve of MP51421-1

    Cytometric bead array standard curve of MP51421-1, SERPINA12 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67573-1-PBS. Detection antibody: 67573-2-PBS. Standard:Ag30136. Range: 0.098-100 ng/mL.

Serpin A12/Vaspin Monoclonal antibody, PBS Only (Capture)

Serpin A12/Vaspin Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 67573-1-PBS

产品说明书

CloneNo. 2A1B6

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

SERPINA12, SERPINA12/Vaspin, Vaspin, 2A1B6, OL 64

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67573-1-PBS targets Serpin A12/Vaspin as part of a matched antibody pair:

MP51421-1: 67573-1-PBS capture and 67573-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag30136

Product name: Recombinant human SERPINA12 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 275-370 aa of BC040857

Sequence: PDEGKLKHLEKGLQVDTFSRWKTLLSRRVVDVSVPRLHMTGTFDLKKTLSYIGVSKIFEEHGDLTKIAPHRSLKVGEAVHKAELKMDERGTEGAAG

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12
别名 SERPINA12, SERPINA12/Vaspin, Vaspin, 2A1B6, OL 64
计算分子量 414 aa, 47 kDa
观测分子量 47 kDa
GenBank蛋白编号BC040857
基因名称 Vaspin
Gene ID (NCBI) 145264
RRIDAB_2882786
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8IW75
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SERPINA12 is also named as Serpin A12 and Visceral adipose tissue-derived serine protease inhibitor (Vaspin). Vaspin is an adipokine belonging to the serpin family, thus also named SERPINA12, with targets in kallikrein 7 and 14 that participates in skin desquamation (PMID: 34359881). Vaspin is a compensatory molecule in obesity and insulin resistance (PMID: 18321232). Vaspin is a newly described adipokine with an insulin sensitizing effect and inhibitory impact on food intake (PMID: 16030142, PMID: 21465327).