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验证数据展示

  • WB analysis using 67573-1-Ig

    WB analysis using 67573-1-Ig

    Human adipose tissue were subjected to SDS PAGE followed by western blot with 67573-1-Ig (Vaspin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 67573-1-Ig

    IF Staining of HeLa using 67573-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Vaspin antibody (67573-1-Ig, Clone: 2A1B6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

Serpin A12/Vaspin Monoclonal antibody

Serpin A12/Vaspin Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67573-1-Ig

产品说明书

CloneNo. 2A1B6

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

SERPINA12, SERPINA12/Vaspin, Vaspin, 2A1B6, OL 64

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman adipose tissue, human placenta tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67573-1-Ig targets Serpin A12/Vaspin in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Serpin A12/Vaspin fusion protein Ag30136 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12
别名 SERPINA12, SERPINA12/Vaspin, Vaspin, 2A1B6, OL 64
计算分子量 414 aa, 47 kDa
观测分子量 47 kDa
GenBank蛋白编号BC040857
基因名称 Vaspin
Gene ID (NCBI) 145264
RRIDAB_2882786
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8IW75
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SERPINA12 is also named as Serpin A12 and Visceral adipose tissue-derived serine protease inhibitor (Vaspin). Vaspin is an adipokine belonging to the serpin family, thus also named SERPINA12, with targets in kallikrein 7 and 14 that participates in skin desquamation (PMID: 34359881). Vaspin is a compensatory molecule in obesity and insulin resistance (PMID: 18321232). Vaspin is a newly described adipokine with an insulin sensitizing effect and inhibitory impact on food intake (PMID: 16030142, PMID: 21465327).

实验方案

Product Specific Protocols
WB protocol for Serpin A12/Vaspin antibody 67573-1-IgDownload protocol
IF protocol for Serpin A12/Vaspin antibody 67573-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols