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验证数据展示

  • WB analysis using 67515-1-Ig

    WB analysis using 67515-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67515-1-Ig (TPI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 67515-1-Ig

    WB analysis of HEK-293 using 67515-1-Ig

    WB result of TPI1 antibody (67515-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPI1 transfected HEK-293 cells.

  • IHC staining of human colon cancer using 67515-1-Ig

    IHC staining of human colon cancer using 67515-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67515-1-Ig (TPI1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TPI1 antibody included
  • IF Staining of HeLa using 67515-1-Ig

    IF Staining of HeLa using 67515-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TPI1 antibody (67515-1-Ig, Clone: 2E7E5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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  • KD/KO验证

TPI1 Monoclonal antibody

TPI1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67515-1-Ig

产品说明书

CloneNo. 2E7E5
引用文献(2)

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat and More (1)

应用

WB, IHC, IF/ICC, ELISA

别名

TIM, TPI, TPI1, Triose phosphate isomerase, Triosephosphate isomerase, triosephosphate isomerase 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TPI1 Ready-To-Use IHC Kit. TPI1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

67515-1-Ig targets TPI1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 Human, Mouse, Rat
文献引用反应性mouse
免疫原

CatNo: Ag16653

Product name: Recombinant human TPI1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-249 aa of BC007086

Sequence: MAPSRKFFVGGNWKMNGRKQSLGELIGTLNAAKVPADTEVVCAPPTAYIDFARQKLDPKIAVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVLGHSERRHVFGESDELIGQKVAHALAEGLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKSNVSDAVAQSTRIIYGGSVTGATCKELASQPDVDGFLVGGASLKPEFVDIINAKQ

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 triosephosphate isomerase 1
别名 TIM, TPI, TPI1, Triose phosphate isomerase, Triosephosphate isomerase, triosephosphate isomerase 1
计算分子量 27 kDa
观测分子量 27 kDa
GenBank蛋白编号BC007086
基因名称 TPI1
Gene ID (NCBI) 7167
RRIDAB_2882736
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP60174
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TPI1(Triosephosphate isomerase) is also named as TIM, TPI and belongs to the triosephosphate isomerase family. It is a key enzyme in cell metabolism that controls the glycolytic flow and energy production through the interconversion of dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde 3-phosphate (G3P)(PMID:19251756). Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). It can exsit as a homodimer(PMID:18562316).

实验方案

Product Specific Protocols
WB protocol for TPI1 antibody 67515-1-IgDownload protocol
IHC protocol for TPI1 antibody 67515-1-IgDownload protocol
IF protocol for TPI1 antibody 67515-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Food Chem

Phosphorylation and acetylation responses of glycolytic enzymes in meat to different chilling rates

Authors - Yuqiang Bai
View Article
mouseWB

Int Immunopharmacol

Candida tropicalis induces NLRP3 inflammasome activation via glycogen metabolism-dependent glycolysis and JAK-STAT1 signaling pathway in myeloid-derived suppressor cells to promote colorectal carcinogenesis

Authors - Zhiyong Zhang
View Article