• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67465-1-Ig

    WB analysis using 67465-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67465-1-Ig (NAT10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 67465-1-Ig

    WB analysis of A549 using 67465-1-Ig

    WB result of NAT10 antibody (67465-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NAT10 transfected A549 cells.

  • Featured Product
  • KD/KO验证

NAT10 Monoclonal antibody

NAT10 Monoclonal Antibody for WB, ELISA
Cat No. 67465-1-Ig

产品说明书

CloneNo. 3E3D8

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

18S rRNA cytosine acetyltransferase, 3E3D8, ALP, EC:2.3.1.-, KIAA1709

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inLNCaP cells, A549 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, Neuro-2a cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67465-1-Ig targets NAT10 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4355

Product name: Recombinant human NAT10 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 676-1025 aa of BC035558

Sequence: EAVSLLEEVITPRKDLPPLLLKLNERPAERLDYLGVSYGLTPRLLKFWKRAGFVPVYLRQTPNDLTGEHSCIMLKTLTDEDEADQGGWLAAFWKDFRRRFLALLSYQFSTFSPSLALNIIQNRNMGKPAQPALSREELEALFLPYDLKRLEMYSRNMVDYHLIMDMIPAISRIYFLNQLGDLALSAAQSALLLGIGLQHKSVDQLEKEIELPSGQLMGLFNRIIRKVVKLFNEVQEKAIEEQMVAAKDVVMEPTMKTLSDDLDEAAKEFQEKHKKEVGKLKSMDLSEYIIRGDDEEWNEVLNKAGPNASIISLKSDKKRKLEAKQEPKQSKKLKNRETKNKKDMKLKRKK

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 N-acetyltransferase 10 (GCN5-related)
别名 18S rRNA cytosine acetyltransferase, 3E3D8, ALP, EC:2.3.1.-, KIAA1709
计算分子量 1025 aa, 116 kDa
观测分子量 116 kDa
GenBank蛋白编号BC035558
基因名称 NAT10
Gene ID (NCBI) 55226
RRIDAB_2882694
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9H0A0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NAT10 (N-acetyltransferase 10) is a nucleolar protein that is involved in regulation of telomerase activity, DNA damage response, and cytokinesis. It also plays a role in maintaining nuclear shape. Inhibition of NAT10 has been reported to rescue the misshapen nuclei in laminopathic cells via microtubule reorganization.

实验方案

Product Specific Protocols
WB protocol for NAT10 antibody 67465-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols