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验证数据展示

  • WB analysis using 67461-1-Ig (same clone as 67461-1-PBS)

    WB analysis using 67461-1-Ig (same clone as 67461-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67461-1-Ig (ARFIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 67461-1-Ig (same clone as 67461-1-PBS)

    IHC staining of mouse kidney using 67461-1-Ig (same clone as 67461-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67461-1-Ig (Arfaptin-1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ARFIP1 antibody included
  • IHC staining of mouse kidney using 67461-1-Ig (same clone as 67461-1-PBS)

    IHC staining of mouse kidney using 67461-1-Ig (same clone as 67461-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67461-1-Ig (Arfaptin-1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ARFIP1 antibody included
  • IF Staining of mouse kidney using 67461-1-Ig (same clone as 67461-1-PBS)

    IF Staining of mouse kidney using 67461-1-Ig (same clone as 67461-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Arfaptin-1 antibody (67461-1-Ig, Clone: 2A10B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse kidney using 67461-1-Ig (same clone as 67461-1-PBS)

    IF Staining of mouse kidney using 67461-1-Ig (same clone as 67461-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Arfaptin-1 antibody (67461-1-Ig, Clone: 2A10B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67461-1-Ig (same clone as 67461-1-PBS)

    IF Staining of HeLa using 67461-1-Ig (same clone as 67461-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Arfaptin-1 antibody (67461-1-Ig, Clone: 2A10B3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 67461-1-Ig (same clone as 67461-1-PBS)

    IF Staining of A549 using 67461-1-Ig (same clone as 67461-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Arfaptin-1 antibody (67461-1-Ig, Clone: 2A10B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51228-2

    Cytometric bead array standard curve of MP51228-2

    Cytometric bead array standard curve of MP51228-2, Arfaptin-1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67461-3-PBS. Detection antibody: 67461-1-PBS. Standard:Ag12097. Range: 3.125-100 ng/mL.

Arfaptin-1 Monoclonal antibody, PBS Only (Detector)

Arfaptin-1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA
Cat No. 67461-1-PBS

产品说明书

CloneNo. 2A10B3

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

别名

ARFIP1, Arfaptin 1, ADP-ribosylation factor-interacting protein 1, 2A10B3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ARFIP1 Ready-To-Use IHC Kit. ARFIP1 primary antibody included.

产品信息

67461-1-PBS targets Arfaptin-1 as part of a matched antibody pair:

MP51228-2: 67461-3-PBS capture and 67461-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12097

Product name: Recombinant human ARFIP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-373 aa of BC103759

Sequence: MAQESPKNSAAEIPVTSNGEVDDSREHSFNRDLKHSLPSGLGLSETQITSHGFDNTKEGVIEAGAFQGSPAPPLPSVMSPSRVAASRLAQQGSDLIVPAGGQRTQTKSGPVILADEIKNPAMEKLELVRKWSLNTYKCTRQIISEKLGRGSRTVDLELEAQIDILRDNKKKYENILKLAQTLSTQLFQMVHTQRQLGDAFADLSLKSLELHEEFGYNADTQKLLAKNGETLLGAINFFIASVNTLVNKTIEDTLMTVKQYESARIEYDAYRTDLEELNLGPRDANTLPKIEQSQHLFQAHKEKYDKMRNDVSVKLKFLEENKVKVLHNQLVLFHNAIAAYFAGNQKQLEQTLKQFHIKLKTPGVDAPSWLEEQ

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 ADP-ribosylation factor interacting protein 1
别名 ARFIP1, Arfaptin 1, ADP-ribosylation factor-interacting protein 1, 2A10B3
计算分子量 373 aa, 42 kDa
观测分子量 38-42 kDa
GenBank蛋白编号BC103759
基因名称 Arfaptin-1
Gene ID (NCBI) 27236
RRIDAB_2882691
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP53367
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C