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验证数据展示

  • IF Staining of COLO 320 using 67258-2-Ig

    IF Staining of COLO 320 using 67258-2-Ig

    Immunofluorescent analysis of (4% PFA) fixed COLO 320 cells using P glycoprotein antibody (67258-2-Ig, Clone: 1G6C2 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

  • WB analysis of HeLa using 67258-2-Ig

    WB analysis of HeLa using 67258-2-Ig

    unboiled HeLa cells were subjected to SDS PAGE followed by western blot with 67258-2-Ig (ABCB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of SH-SY5Y using 67258-2-Ig

    WB analysis of SH-SY5Y using 67258-2-Ig

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67258-2-Ig (ABCB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 67258-2-Ig

    WB analysis of HepG2 using 67258-2-Ig

    unboiled HepG2 cells were subjected to SDS PAGE followed by western blot with 67258-2-Ig (ABCB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 67258-2-Ig

    IHC staining of human kidney using 67258-2-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67258-2-Ig (ABCB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ABCB1 antibody included
  • IHC staining of human liver using 67258-2-Ig

    IHC staining of human liver using 67258-2-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67258-2-Ig (ABCB1 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ABCB1 antibody included

P glycoprotein Monoclonal antibody

P glycoprotein Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67258-2-Ig

产品说明书

CloneNo. 1G6C2
引用文献(1)

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

ABCB1, 1G6C2, EC:7.6.2.2, EC:7.6.2.1, ATP-dependent translocase ABCB1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inunboiled HeLa cells, unboiled HepG2 cells, SH-SY5Y cells
Positive IHC detected inhuman kidney tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inCOLO 320 cells

Boiling the sample after lysis is not recommended.

Planning an IHC experiment? We recommend our IHCeasy ABCB1 Ready-To-Use IHC Kit. ABCB1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67258-2-Ig targets P glycoprotein in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag17811

Product name: Recombinant human ABCB1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 624-708 aa of BC130424

Sequence: KLVTMQTAGNEVELENAADESKSEIDALEMSSNDSRSSLIRKRSTRRSVRGSQAQDRKLSTKEALDESIPPVSFWRIMKLNLTEW

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ATP-binding cassette, sub-family B (MDR/TAP), member 1
别名 ABCB1, 1G6C2, EC:7.6.2.2, EC:7.6.2.1, ATP-dependent translocase ABCB1
计算分子量 1280 aa, 142 kDa
观测分子量130-150 kDa
GenBank蛋白编号BC130424
基因名称 P glycoprotein
Gene ID (NCBI) 5243
RRIDAB_3085039
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP08183
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ABCB1 (also known as P-gp/ P-glycoprotein, MDR1/multidrug-resistance 1) is a plasma membrane protein first discovered in multidrug resistant tumor cells. ABCB1 can extrude a large number of medically relevant compounds from cells and causes drug resistance. ABCB1 is expressed in a variety of human cancers as well as normal tissues including brain, and placenta. Overexpression of ABCB1 correlates with a negative prognosis in several types of cancer. For optimal WB detection, we recommend to avoid boiling the sample after lysis.

实验方案

Product Specific Protocols
WB protocol for P glycoprotein antibody 67258-2-IgDownload protocol
IHC protocol for P glycoprotein antibody 67258-2-IgDownload protocol
IF protocol for P glycoprotein antibody 67258-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Drug Resist Updat

YTHDF2-mediated m6A modification of ONECUT2 promotes stemness and oxaliplatin resistance in gastric cancer through transcriptionally activating TFPI

Authors - Xingdi Fan
View Article