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验证数据展示

  • IHC staining of human lung cancer using 67239-1-Ig (same clone as 67239-1-PBS)

    IHC staining of human lung cancer using 67239-1-Ig (same clone as 67239-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67239-1-Ig (CDC27; APC3 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDC27 antibody included
  • IHC staining of human lung cancer using 67239-1-Ig (same clone as 67239-1-PBS)

    IHC staining of human lung cancer using 67239-1-Ig (same clone as 67239-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67239-1-Ig (CDC27; APC3 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDC27 antibody included
  • WB analysis of Jurkat using 67239-1-Ig (same clone as 67239-1-PBS)

    WB analysis of Jurkat using 67239-1-Ig (same clone as 67239-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67239-1-Ig (CDC27; APC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51673-1

    Cytometric bead array sample test of MP51673-1

    Cytometric bead array sample test of MP51673-1, CDC27 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67239-1-PBS. Detection antibody: 67239-2-PBS.

  • FC experiment of K-562 using 67239-1-Ig (same clone as 67239-1-PBS)

    FC experiment of K-562 using 67239-1-Ig (same clone as 67239-1-PBS)

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human CDC27; APC3 (67239-1-Ig, Clone:2F10G2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

  • IF Staining of human lung cancer using 67239-1-Ig (same clone as 67239-1-PBS)

    IF Staining of human lung cancer using 67239-1-Ig (same clone as 67239-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CDC27; APC3 antibody (67239-1-Ig, Clone: 2F10G2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

  • IF Staining of human lung cancer using 67239-1-Ig (same clone as 67239-1-PBS)

    IF Staining of human lung cancer using 67239-1-Ig (same clone as 67239-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CDC27; APC3 antibody (67239-1-Ig, Clone: 2F10G2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 67239-1-Ig (same clone as 67239-1-PBS)

    IF Staining of HEK-293 using 67239-1-Ig (same clone as 67239-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CDC27; APC3 antibody (67239-1-Ig, Clone: 2F10G2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51673-1

    Cytometric bead array standard curve of MP51673-1

    Cytometric bead array standard curve of MP51673-1, CDC27 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67239-1-PBS. Detection antibody: 67239-2-PBS. Standard:Ag25588. Range: 0.781-100 ng/mL.

CDC27; APC3 Monoclonal antibody, PBS Only (Capture)

CDC27; APC3 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 67239-1-PBS

产品说明书

CloneNo. 2F10G2

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA

别名

CDC27, ANAPC3, Anaphase-promoting complex subunit 3, APC 3, APC3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CDC27 Ready-To-Use IHC Kit. CDC27 primary antibody included.

产品信息

67239-1-PBS targets CDC27; APC3 as part of a matched antibody pair:

MP51673-1: 67239-1-PBS capture and 67239-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag25588

Product name: Recombinant human CDC27; APC3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 481-830 aa of BC011656

Sequence: ALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 cell division cycle 27 homolog (S. cerevisiae)
别名 CDC27, ANAPC3, Anaphase-promoting complex subunit 3, APC 3, APC3
计算分子量 92 kDa
观测分子量 90-100 kDa
GenBank蛋白编号BC011656
基因名称 CDC27
Gene ID (NCBI) 996
RRIDAB_2882518
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP30260
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CDC27/APC3 is a component of the anaphase-promoting complex (APC/cyclosome), which is composed of eight subunits and highly conserved in eukaryotic cells. The APC/cyclosome complex acts as a cell cycle-regulated E3 ubiquitin ligase which mediates ubiquitination and subsequent degradation of target proteins, and lead to the progression control through mitosis and the G1 phase of the cell cycle.

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