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验证数据展示

  • IHC staining of human pituitary using 67131-1-Ig

    IHC staining of human pituitary using 67131-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 67131-1-Ig (FSH beta antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pituitary using 67131-1-Ig

    IHC staining of human pituitary using 67131-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 67131-1-Ig (FSHB antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 67131-1-Ig

    IF Staining of HepG2 using 67131-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FSH beta antibody (67131-1-Ig, Clone: 1B10A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

FSH beta Monoclonal antibody

FSH beta Monoclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 67131-1-Ig

产品说明书

CloneNo. 1B10A11

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

IHC, IF/ICC, ELISA

别名

FSHB, 1B10A11, Follicle-stimulating hormone beta subunit, Follitropin beta chain, Follitropin subunit beta

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IHC detected inhuman pituitary tissue, human pituitary adenoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67131-1-Ig targets FSH beta in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag13788

Product name: Recombinant human FSHB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 19-129 aa of BC113488

Sequence: NSCELTNITIAIEKEECRFCISINTTWCAGYCYTRDLVYKDPARPKIQKTCTFKELVYETVRVPGCAHHADSLYTYPVATQCHCGKCDSDSTDCTVRGLGPSYCSFGEMKE

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 follicle stimulating hormone, beta polypeptide
别名 FSHB, 1B10A11, Follicle-stimulating hormone beta subunit, Follitropin beta chain, Follitropin subunit beta
计算分子量 129 aa, 15 kDa
GenBank蛋白编号BC113488
基因名称 FSHB
Gene ID (NCBI) 2488
RRIDAB_2882430
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP01225
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The FSHB gene encodes the beta subunit of follicle-stimulating hormone (FSH), particularly expressed in gonadotroph cells within the anterior pituitary gland and plays a critical role in the regulation of gonadal function, pubertal maturation and reproductive processes in mammals. The transcription of the FSHB gene is critical for the production of the FSH hormone(PMID:28281143). And FSH is required for ovarian folliculogenesis in females, in males it promotes spermatogenesis in conjunction with testosterone(PMID:11739331). Mutations in FSHB gene would result in infertility both in men and women (PMID: 23766128).

实验方案

Product Specific Protocols
IF protocol for FSH beta antibody 67131-1-IgDownload protocol
IHC protocol for FSH beta antibody 67131-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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