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验证数据展示

  • IHC staining of human colon cancer using 67037-1-Ig (same clone as 67037-1-PBS)

    IHC staining of human colon cancer using 67037-1-Ig (same clone as 67037-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67037-1-Ig (KDM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67037-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KDM1 antibody included
  • WB analysis using 67037-1-Ig (same clone as 67037-1-PBS)

    WB analysis using 67037-1-Ig (same clone as 67037-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67037-1-Ig (KDM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67037-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51540-1

    Cytometric bead array sample test of MP51540-1

    Cytometric bead array sample test of MP51540-1, KDM1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67037-1-PBS. Detection antibody: 67037-2-PBS.

  • Cytometric bead array standard curve of MP51540-1

    Cytometric bead array standard curve of MP51540-1

    Cytometric bead array standard curve of MP51540-1, KDM1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67037-1-PBS. Detection antibody: 67037-2-PBS. Standard:Ag14778. Range: 3.125-400 ng/mL.

KDM1 Monoclonal antibody, PBS Only (Capture)

KDM1 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 67037-1-PBS

产品说明书

CloneNo. 2F10A12

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, Cytometric bead array, Indirect ELISA

别名

LSD1, [histone H3]-dimethyl-L-lysine(4) FAD-dependent demethylase 1A, 2F10A12, AOF 2, AOF2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy KDM1 Ready-To-Use IHC Kit. KDM1 primary antibody included.

产品信息

67037-1-PBS targets KDM1 as part of a matched antibody pair:

MP51540-1: 67037-1-PBS capture and 67037-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag14778

Product name: Recombinant human KDM1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 524-876 aa of BC040194

Sequence: TQGKLEEKLQELEANPPSDVYLSSRDRQILDWHFANLEFANATPLSTLSLKHWDQDDDFEFTGSHLTVRNGYSCVPVALAEGLDIKLNTAVRQVRYTASGCEVIAVNTRSTSQTFIYKCDAVLCTLPLGVLKQQPPAVQFVPPLPEWKTSAVQRMGFGNLNKVVLCFDRVFWDPSVNLFGHVGSTTASRGELFLFWNLYKAPILLALVAGEAAGIMENISDDVIVGRCLAILKGIFGSSAVPQPKETVVSRWRADPWARGSYSYVAAGSSGNDYDLMAQPITPGPSIPGAPQPIPRLFFAGEHTIRNYPATVHGALLSGLREAGRIADQFLGAMYTLPRQATPGVPAQQSPSM

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 lysine (K)-specific demethylase 1
别名 LSD1, [histone H3]-dimethyl-L-lysine(4) FAD-dependent demethylase 1A, 2F10A12, AOF 2, AOF2
计算分子量 876 aa, 95 kDa
观测分子量 110 kDa
GenBank蛋白编号BC040194
基因名称 KDM1
Gene ID (NCBI) 23028
RRIDAB_2882351
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDO60341
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Lysine specific demethylase 1 (LSD1/BHC110/KIAA0601/p110b/AOF2/KDM1) is an amine oxidase that catalyzes histone demethylation via a flavin adenine dinucleotide (FAD)-dependent oxidative reaction(PMID:19703393). This protein belongs to the flavin monoamine oxidase family. Its structure and function is conserved from yeast to human and it is typically associated to CoREST, a corepressor protein, and histone deacetylases HDAC1 and HDAC2. Human LSD1 consists of 852 amino acids and comprises an N-terminal SWIRM domain, involved in protein interactions, and a C-terminal amine oxidase domain, which contains an insertion that forms the CoREST interacting site (the so-called tower domain )(PMID:20164337). It has 2 isoforms produced by alternative splicing with the calculated molecular mass of 93-95 kDa and apparent molecular mass of 110 kDa.

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