验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells, HepG2 cells, PC-12 cells, 4T1 cells |
| Positive IHC detected in | human colon cancer tissue, human lung cancer tissue, human liver cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
67037-1-Ig targets KDM1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human, Mouse, Rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag14778 Product name: Recombinant human KDM1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 524-876 aa of BC040194 Sequence: TQGKLEEKLQELEANPPSDVYLSSRDRQILDWHFANLEFANATPLSTLSLKHWDQDDDFEFTGSHLTVRNGYSCVPVALAEGLDIKLNTAVRQVRYTASGCEVIAVNTRSTSQTFIYKCDAVLCTLPLGVLKQQPPAVQFVPPLPEWKTSAVQRMGFGNLNKVVLCFDRVFWDPSVNLFGHVGSTTASRGELFLFWNLYKAPILLALVAGEAAGIMENISDDVIVGRCLAILKGIFGSSAVPQPKETVVSRWRADPWARGSYSYVAAGSSGNDYDLMAQPITPGPSIPGAPQPIPRLFFAGEHTIRNYPATVHGALLSGLREAGRIADQFLGAMYTLPRQATPGVPAQQSPSM 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | lysine (K)-specific demethylase 1 |
| 别名 | AOF2, BHC110, KDM1, KDM1A, KIAA0601, LSD1, lysine (K) demethylase 1 |
| 计算分子量 | 876 aa, 95 kDa |
| 观测分子量 | 110 kDa |
| GenBank蛋白编号 | BC040194 |
| 基因名称 | KDM1 |
| Gene ID (NCBI) | 23028 |
| RRID | AB_2882351 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | O60341 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Lysine specific demethylase 1 (LSD1/BHC110/KIAA0601/p110b/AOF2/KDM1) is an amine oxidase that catalyzes histone demethylation via a flavin adenine dinucleotide (FAD)-dependent oxidative reaction(PMID:19703393). This protein belongs to the flavin monoamine oxidase family. Its structure and function is conserved from yeast to human and it is typically associated to CoREST, a corepressor protein, and histone deacetylases HDAC1 and HDAC2. Human LSD1 consists of 852 amino acids and comprises an N-terminal SWIRM domain, involved in protein interactions, and a C-terminal amine oxidase domain, which contains an insertion that forms the CoREST interacting site (the so-called tower domain )(PMID:20164337). It has 2 isoforms produced by alternative splicing with the calculated molecular mass of 93-95 kDa and apparent molecular mass of 110 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for KDM1 antibody 67037-1-Ig | Download protocol |
| WB protocol for KDM1 antibody 67037-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
