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验证数据展示

  • WB analysis of A549 using 66914-1-Ig (same clone as 66914-1-PBS)

    WB analysis of A549 using 66914-1-Ig (same clone as 66914-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 66914-1-Ig (Cathepsin L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66914-1-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 66914-1-Ig (same clone as 66914-1-PBS)

    WB analysis of A549 using 66914-1-Ig (same clone as 66914-1-PBS)

    WB result of Cathepsin L antibody (66914-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cathepsin L transfected A549 cells. This data was developed using the same antibody clone with 66914-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 66914-1-Ig (same clone as 66914-1-PBS)

    IHC staining of human prostate cancer using 66914-1-Ig (same clone as 66914-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66914-1-Ig (Cathepsin L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66914-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CTSL1 antibody included
  • IF Staining of HEK-293 using 66914-1-Ig (same clone as 66914-1-PBS)

    IF Staining of HEK-293 using 66914-1-Ig (same clone as 66914-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using Cathepsin L antibody (66914-1-Ig, Clone: 1A12G8 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66914-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51216-1

    Cytometric bead array standard curve of MP51216-1

    Cytometric bead array standard curve of MP51216-1, Cathepsin L Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66914-2-PBS. Detection antibody: 66914-1-PBS. Standard:Ag27951. Range: 0.781-50 ng/mL.

  • Affinity and Kinetic Characterization of 66914-1-Ig

    Affinity and Kinetic Characterization of 66914-1-Ig

    Biolayer interferometry (BLl) kinetic assay of 66914-1-Ig against Human Cathepsin L was performed. The affinity constant is 4.84 nM.

  • Featured Product
  • KD/KO验证

Cathepsin L Monoclonal antibody, PBS Only (Detector)

Cathepsin L Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 66914-1-PBS

产品说明书

CloneNo. 1A12G8

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

CTSL1, 1A12G8, Cathepsin L heavy chain, Cathepsin L light chain, cathepsin L1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CTSL1 Ready-To-Use IHC Kit. CTSL1 primary antibody included.

产品信息

66914-1-PBS targets Cathepsin L as part of a matched antibody pair:

MP51216-1: 66914-2-PBS capture and 66914-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag27951

Product name: Recombinant human Cathepsin L protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-333 aa of BC012612

Sequence: MNPTLILAAFCLGIASATLTFDHSLEAQWTKWKAMHNRLYGMNEEGWRRAVWEKNVKMIELHNQEYREGKHSFTMAMNAFGDMTSEEFRQVMNGFQNRKPRKGKVFQEPLFYEAPRSVDWREKGYVTPVKNQGQCGSCWAFSATGALEGQMFRKTGRLISLSEQNLVDCSGPQGNEGCNGGLMDYAFQYVQDNGGLDSEESYPYEATEESCKYNPKYSVANDTGFVDIPKQEKALMKAVATVGPISVAIDAGHESFLFYKEGIYFEPDCSSEDMDHGVLVVGYGFESTESDNNKYWLVKNSWGEEWGMGGYVKMAKDRRNHCGIASAASYPTV

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 cathepsin L1
别名 CTSL1, 1A12G8, Cathepsin L heavy chain, Cathepsin L light chain, cathepsin L1
计算分子量 38 kDa
观测分子量43 kDa
GenBank蛋白编号BC012612
基因名称 Cathepsin L
Gene ID (NCBI) 1514
RRIDAB_2882241
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP07711
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CTSL1(Cathepsin L1) is also named as CTSL and MEP, belongs to the peptidase C1 family. It is a lysosomal proteinase whose expression is also up-regulated in the skeletal muscle during starvation(PMID:20088826). It plays an intracellular role in normal intestinal epithelial polarization and initiation of neoplasia(PMID:17622569). CTSL1 also improves cardiac function and inhibits cardiac hypertrophy, inflammation, and fibrosis through blocking AKT/GSK3B signaling(PMID:19096818). The full length protein is 38 kDa with a signal peptide, two propeptide and a glycosylation site. It has been detected the 36 kDa, 39 kDa, 29 kDa and 21 kDa in rat gastrocnemius muscle. These forms of cathepsin L could either be attributed to differences in glycosylation or to partial processing of the proenzyme. (PMID:11696001)

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