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验证数据展示

  • WB analysis of human plasma using 66908-1-Ig

    WB analysis of human plasma using 66908-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66908-1-Ig (C7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 66908-1-Ig

    IF Staining of HepG2 using 66908-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using C7 antibody (66908-1-Ig, Clone: 1H9C4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

  • FC experiment of HepG2 using 66908-1-Ig

    FC experiment of HepG2 using 66908-1-Ig

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug C7 Monoclonal antibody (66908-1-Ig, Clone:1H9C4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

C7 Monoclonal antibody

C7 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66908-1-Ig

产品说明书

CloneNo. 1H9C4
引用文献(1)

宿主/亚型

Mouse / IgG1

种属反应性

human and More (1)

应用

WB, IF/ICC, ELISA and More (1)

别名

Complement component C7, complement component 7, 1H9C4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman plasma tissue
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

66908-1-Ig targets C7 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用IF
经测试反应性 human
文献引用反应性pig
免疫原 C7 fusion protein Ag12552 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 complement component 7
别名 Complement component C7, complement component 7, 1H9C4
计算分子量 843 aa, 94 kDa
观测分子量 100 kDa
GenBank蛋白编号BC063851
基因名称 C7
Gene ID (NCBI) 730
RRIDAB_2882235
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP10643
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C7, a single-chain plasma glycoprotein, is a component of the complement system. It is a constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C7 serves as a membrane anchor. People with C7 deficiency are prone to bacterial infection.

实验方案

Product Specific Protocols
WB protocol for C7 antibody 66908-1-IgDownload protocol
IF protocol for C7 antibody 66908-1-IgDownload protocol
FC protocol for C7 antibody 66908-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
pigIF

Front Cell Dev Biol

Deciphering transcriptome patterns in porcine mesenchymal stem cells promoting phenotypic maintenance and differentiation by key driver genes

Authors - Nadia Khaveh
View Article