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验证数据展示

  • WB analysis using 66775-1-Ig (same clone as 66775-1-PBS)

    WB analysis using 66775-1-Ig (same clone as 66775-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66775-1-Ig (Syntaxin 16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66775-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66775-1-Ig (same clone as 66775-1-PBS)

    IHC staining of human lung cancer using 66775-1-Ig (same clone as 66775-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66775-1-Ig (Syntaxin 16 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66775-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66775-1-Ig (same clone as 66775-1-PBS)

    IHC staining of human lung cancer using 66775-1-Ig (same clone as 66775-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66775-1-Ig (Syntaxin 16 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66775-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66775-1-Ig (same clone as 66775-1-PBS)

    IF Staining of HepG2 using 66775-1-Ig (same clone as 66775-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66775-1-Ig (Syntaxin 16 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66775-1-PBS in a different storage buffer formulation.

  • Sample test of MP51331-1

    Sample test of MP51331-1

    Sample test of MP51331-1, STX16 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66775-1-PBS. Detection antibody: 66775-2-PBS.

  • IF Staining of Neuro-2a using 66775-1-Ig (same clone as 66775-1-PBS)

    IF Staining of Neuro-2a using 66775-1-Ig (same clone as 66775-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using Syntaxin 16 antibody (66775-1-Ig, Clone: 4A9D8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66775-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51331-1

    Cytometric bead array standard curve of MP51331-1

    Cytometric bead array standard curve of MP51331-1, STX16 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66775-1-PBS. Detection antibody: 66775-2-PBS. Standard:Ag27926. Range: 1.563-200 ng/mL.

Syntaxin 16 Monoclonal antibody, PBS Only (Capture)

Syntaxin 16 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test
Cat No. 66775-1-PBS

产品说明书

CloneNo. 4A9D8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test

别名

Syntaxin-16, 4A9D8, STX16, Syn16

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66775-1-PBS targets Syntaxin 16 as part of a matched antibody pair:

MP51331-1: 66775-1-PBS capture and 66775-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse, rat
免疫原 Syntaxin 16 fusion protein Ag27926 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 syntaxin 16
别名 Syntaxin-16, 4A9D8, STX16, Syn16
计算分子量 37 kDa
观测分子量 37-40 kDa
GenBank蛋白编号BC019042
基因名称 Syntaxin 16
Gene ID (NCBI) 8675
RRIDAB_2882121
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO14662
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Syntaxin 16 (STX16), also named as SYN16, belongs to the syntaxin family. Syntaxins are target membrane SNAREs(Soluble NSF attachment protein receptors) which are membrane components that determine the specificity of the docking and fusion of vesicles to target membranes. STX16 is localized to the Golgi apparatus.