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验证数据展示

  • WB analysis of human adipose using 66641-1-Ig

    WB analysis of human adipose using 66641-1-Ig

    human adipose tissue were subjected to SDS PAGE followed by western blot with 66641-1-Ig (ANGPTL8/Betatrophin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 66641-1-Ig

    IHC staining of human liver using 66641-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66641-1-Ig (ANGPTL8/Betatrophin antibody) at dilution of 1:400 (under 40x lens).

  • IHC staining of human liver using 66641-1-Ig

    IHC staining of human liver using 66641-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66641-1-Ig (ANGPTL8/Betatrophin antibody) at dilution of 1:400 (under 10x lens).

  • IF Staining of human liver using 66641-1-Ig

    IF Staining of human liver using 66641-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using ANGPTL8/Betatrophin antibody (66641-1-Ig, Clone: 2H9F9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

ANGPTL8/Betatrophin Monoclonal antibody

ANGPTL8/Betatrophin Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66641-1-Ig

产品说明书

CloneNo. 2H9F9
引用文献(1)

宿主/亚型

Mouse / IgG1

种属反应性

Human and More (1)

应用

WB, IHC, IF-P, ELISA

别名

Angiopoietin-like protein 8, ANGPTL8, Betatrophin, C19orf80, Lipasin, LOC55908, PRO1185, PVPA599, td26

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite®594
规格价格库存

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经过测试的应用

Positive WB detected inhuman adipose tissue
Positive IHC detected inhuman liver tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver tissue
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66641-1-Ig targets ANGPTL8/Betatrophin in WB, IHC, IF-P, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB
经测试反应性 Human
文献引用反应性human
免疫原

CatNo: Ag20708

Product name:

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: of

Sequence:

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 hepatocellular carcinoma-associated gene TD26
别名 Angiopoietin-like protein 8, ANGPTL8, Betatrophin, C19orf80, Lipasin, LOC55908, PRO1185, PVPA599, td26
计算分子量 251 aa, 28 kDa
观测分子量 22 kDa
GenBank蛋白编号BC146552
基因名称 ANGPTL8
Gene ID (NCBI) 55908
RRIDAB_2882000
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ6UXH0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ANGPTL8/Betatrophin antibody 66641-1-IgDownload protocol
IHC protocol for ANGPTL8/Betatrophin antibody 66641-1-IgDownload protocol
IF protocol for ANGPTL8/Betatrophin antibody 66641-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Br J Pharmacol

Sodium rutin extends lifespan and health span in mice including positive impacts on liver health.

Authors - Shuoshuo Li
View Article