验证数据展示
经过测试的应用
| Positive WB detected in | HUVEC cells |
| Positive IF/ICC detected in | HUVEC cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1500-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
66537-1-Ig targets EXOC3L2 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human |
| 文献引用反应性 | human |
| 免疫原 | EXOC3L2 fusion protein Ag26912 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | exocyst complex component 3-like 2 |
| 别名 | EXOC3L2, XTP7 |
| 计算分子量 | 46 kDa |
| 观测分子量 | 46 kDa |
| GenBank蛋白编号 | BC104947 |
| 基因名称 | EXOC3L2 |
| Gene ID (NCBI) | 90332 |
| RRID | AB_2881900 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q2M3D2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The exocyst is a protein complex that ensures spatial targeting of exocytotic vesicles to the plasma membrane. Exocyst complex component 3-like 2 (EXOC3L2) associates with the exocyst complex and mediates directional migration of endothelial cells (PMID: 21566143). The gene of human EXOC3L2 maps to chromosome 19q13.32 and encodes a deduced 409-amino acid protein with a calculated molecular mass of about 46 kDa. Mouse Exoc3l2 gene encodes an approximately 250-amino acid protein that lacks the N-terminal domain of human EXOC3L2. Mutations in EXOC3L2 gene may be associated with Alzheimer's disease.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EXOC3L2 antibody 66537-1-Ig | Download protocol |
| IF protocol for EXOC3L2 antibody 66537-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |