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验证数据展示

  • WB analysis using 66497-1-Ig (same clone as 66497-1-PBS)

    WB analysis using 66497-1-Ig (same clone as 66497-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66497-1-Ig (CCAR2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51483-1

    Cytometric bead array sample test of MP51483-1

    Cytometric bead array sample test of MP51483-1, CCAR2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66497-2-PBS. Detection antibody: 66497-1-PBS.

  • WB analysis of HEK-293 using 66497-1-Ig (same clone as 66497-1-PBS)

    WB analysis of HEK-293 using 66497-1-Ig (same clone as 66497-1-PBS)

    WB result of CCAR2 antibody (66497-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCAR2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 66497-1-Ig (same clone as 66497-1-PBS)

    IHC staining of human cervical cancer using 66497-1-Ig (same clone as 66497-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DBC1/CCAR2 antibody included
  • IHC staining of human cervical cancer using 66497-1-Ig (same clone as 66497-1-PBS)

    IHC staining of human cervical cancer using 66497-1-Ig (same clone as 66497-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DBC1/CCAR2 antibody included
  • IF Staining of HeLa using 66497-1-Ig (same clone as 66497-1-PBS)

    IF Staining of HeLa using 66497-1-Ig (same clone as 66497-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66497-1-Ig (CCAR2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 66497-1-Ig (same clone as 66497-1-PBS)

    FC experiment of U2OS using 66497-1-Ig (same clone as 66497-1-PBS)

    1X10^6 U2OS cells were intracellularly stained with 0.2 ug Anti-Human CCAR2 (66497-1-Ig, Clone:2D10F8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66497-1-Ig (same clone as 66497-1-PBS)

    IF Staining of HeLa using 66497-1-Ig (same clone as 66497-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CCAR2 antibody (66497-1-Ig, Clone: 2D10F8 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51483-1

    Cytometric bead array standard curve of MP51483-1

    Cytometric bead array standard curve of MP51483-1, CCAR2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66497-2-PBS. Detection antibody: 66497-1-PBS. Standard:Ag18374. Range: 1.563-100 ng/mL.

  • Featured Product
  • KD/KO验证

CCAR2 Monoclonal antibody, PBS Only (Detector)

CCAR2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 66497-1-PBS

产品说明书

CloneNo. 2D10F8

宿主/亚型

Mouse / IgG2a

种属反应性

human, pig

应用

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

cell cycle and apoptosis regulator 2, DBC1, DBC-1, KIAA1967, Cell cycle and apoptosis regulator protein 2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy DBC1/CCAR2 Ready-To-Use IHC Kit. DBC1/CCAR2 primary antibody included.

产品信息

66497-1-PBS targets CCAR2 as part of a matched antibody pair:

MP51483-1: 66497-2-PBS capture and 66497-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, pig
免疫原

CatNo: Ag18374

Product name: Recombinant human DBC1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 571-923 aa of BC018269

Sequence: EPEKEEAAKEEATKEEEAIKEEVVKEPKDEAQNEGPATESEAPLKEDGLLPKPLSSGGEEEEKPRGEASEDLCEMALDPELLLLRDDGEEEFAGAKLEDSEVRSVASNQSEMEFSSLQDMPKELDPSAVLPLDCLLAFVFFDANWCGYLHRRDLERILLTLGIRLSAEQAKQLVSRVVTQNICQYRSLQYSRQEGLDGGLPEEVLFGNLDLLPPPGKSTKPGAAPTEHKALVSHNGSLINVGSLLQRAEQQDSGRLYLENKIHTLELKLEESHNRFSATEVTNKTLAAEMQELRVRLAEAEETARTAERQKSQLQRLLQELRRRLTPLQLEIQRVVEKADSWVEKEEPAPSN

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 KIAA1967
别名 cell cycle and apoptosis regulator 2, DBC1, DBC-1, KIAA1967, Cell cycle and apoptosis regulator protein 2
计算分子量 923 aa, 103 kDa
观测分子量 130 kDa
GenBank蛋白编号BC018269
基因名称 CCAR2
Gene ID (NCBI) 57805
RRIDAB_2881861
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8N163
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cell cycle and apoptosis regulator protein 2 (CCAR2), previously known as DBC1/KIAA1967, regulates diverse cellular functions including transcription, mRNA splicing, metabolism, and circadian cycle. CCAR2 is a critical regulator of cell death or survival following cellular stresses. CCAR2 is a widely expressed protein involved in the regulation of a variety of transcriptional complexes. High expression of CCAR2 correlates with poor outcomes in many human tumor types such as squamous cell carcinoma (SCC).

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