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验证数据展示

  • FC experiment of human PBMCs using 65103-1-Ig (same clone as 65103-1-PBS)

    FC experiment of human PBMCs using 65103-1-Ig (same clone as 65103-1-PBS)

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD40 (65103-1-Ig, Clone:G28.5) or Mouse IgG1 Isotype Control and CoraLite®647-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CoraLite®488 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65103-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 65103-1-Ig (same clone as 65103-1-PBS)

    FC experiment of human PBMCs using 65103-1-Ig (same clone as 65103-1-PBS)

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD40 (65103-1-Ig, Clone:G28.5) or Mouse IgG1 Isotype Control and CoraLite®647-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65103-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50064-1

    Sandwich ELISA standard curve of MP50064-1

    Sandwich ELISA standard curve of MP50064-1, CD40 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66965-2-PBS. Detection antibody: 65103-1-PBS. Standard: Ag28040. Range: 78.1-5000 pg/mL.

  • Sample test of MP50064-1

    Sample test of MP50064-1

    Urine of eight individual healthy human donors was measured. The CD40 concentration of detected samples was determined to be 1,053.4 pg/mL with a range of 79.9-2,169.7 pg/mL.

  • Sample test of MP50064-1

    Sample test of MP50064-1

    Saliva of six individual healthy human donors was measured. The CD40 concentration of detected samples was determined to be 684.6 pg/mL with a range of 349.9-1,498.5 pg/mL.

  • Sample test of MP50064-1

    Sample test of MP50064-1

    Human milk of six individual healthy human donors was measured. The CD40 concentration of detected samples was determined to be 53.4 pg/mL with a range of 29.8-104.3 pg/mL.

  • Sample test of MP50064-1

    Sample test of MP50064-1

    Plasma of fifteen individual healthy human donors was measured. The CD40 concentration of detected samples was determined to be 89.6 pg/mL with a range of 39.1-155.7 pg/mL.

Anti-Human CD40 (G28.5), PBS Only (Detector)

CD40 Monoclonal Antibody for FC, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 65103-1-PBS

产品说明书

CloneNo. G28.5

宿主/亚型

Mouse / IgG1, kappa

种属反应性

human

应用

FC, Sandwich ELISA, Indirect ELISA, Sample test

别名

B cell surface antigen CD40, B-cell surface antigen CD40, Bp50, CD40L receptor, G28.5

缓冲液配方:  PBS Only
PBS, Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

65103-1-PBS targets CD40 as part of a matched antibody pair:

MP50064-1: 66965-2-PBS capture and 65103-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 FC, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原

N/A

种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 CD40 molecule, TNF receptor superfamily member 5
别名 B cell surface antigen CD40, B-cell surface antigen CD40, Bp50, CD40L receptor, G28.5
计算分子量 277 aa, 31 kDa
GenBank蛋白编号BC012419
基因名称 CD40
Gene ID (NCBI) 958
ENSEMBL Gene IDENSG00000101017
RRIDAB_2881471
偶联类型 Unconjugated
形式Liquid
纯化方式Affinity purification
UNIPROT IDP25942
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD40, also named as TNFRSF5 and Bp50, is a type I transmembrane protein that belongs to the tumor necrosis factor-R (TNF-R) family (PMID: 10647992). CD40 is expressed on B cells, dendritic cells (DCs), monocytes, platelets, and macrophages as well as by non-hematopoietic cells such as myofibroblasts, fibroblasts, epithelial, and endothelial cells (PMID: 19426221). It is a receptor for TNFSF5/CD40LG. CD40L/CD40 interactions are essential for immunoglobulin (Ig) isotype switching, germinal center formation and the development of B cell memory (PMID: 7516405; 11675497).

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