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验证数据展示

  • IF Staining of human tonsillitis using 60270-1-Ig

    IF Staining of human tonsillitis using 60270-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-28A antibody (60270-1-Ig, Clone: 3B4A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human tonsillitis using 60270-1-Ig

    IF Staining of human tonsillitis using 60270-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-28A antibody (60270-1-Ig, Clone: 3B4A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of human tonsillitis using 60270-1-Ig

    IHC staining of human tonsillitis using 60270-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60270-1-Ig (IL-28A antibody at dilution of 1:50 (under 10x lens).

  • IHC staining of human tonsillitis using 60270-1-Ig

    IHC staining of human tonsillitis using 60270-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60270-1-Ig (IL-28A antibody at dilution of 1:50 (under 40x lens).

IL-28A Monoclonal antibody

IL-28A Monoclonal Antibody for IHC, IF-P, ELISA
Cat No. 60270-1-Ig

产品说明书

CloneNo. 3B4A1

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IHC, IF-P, ELISA

别名

IL28A, Cytokine Zcyto20, IFN lambda 2, IFNL2, IFN-lambda-2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman tonsillitis tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60270-1-Ig targets IL-28A in IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag18525

Product name: Recombinant human IL-28A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 21-91 aa of BC113583

Sequence: TVTGAVPVARLHGALPDARGCHIAQFKSLSPQELQAFKRAKDALEESLLLKDCRCHSRLFPRTWDLRQLQV

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
亲和力
KD=1.77 x 109M
KOff=4.10 x 10-5M
KOn=2.31 x 104M
全称 interleukin 28A (interferon, lambda 2)
别名 IL28A, Cytokine Zcyto20, IFN lambda 2, IFNL2, IFN-lambda-2
计算分子量 200 aa, 22 kDa
GenBank蛋白编号BC113583
基因名称 IL-28A
Gene ID (NCBI) 282616
RRIDAB_2881390
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ8IZJ0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IL28A, also named as IFNL2 and ZCYT020, is a cytokine with immunomodulatory activity. It up-regulates MHC class I antigen expression. IL28A is a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1. The ligand/receptor complex seems to signal through the Jak-STAT pathway. It plays a significant role in the antiviral immune defense in the intestinal epithelium.

实验方案

Product Specific Protocols
IHC protocol for IL-28A antibody 60270-1-IgDownload protocol
IF protocol for IL-28A antibody 60270-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols